Article No
515-605-072
Application | IF, FC |
Article No | 515-605-072 |
Country Availability | SE, FI, DK, NO, IS, EE, LV, LT |
Clone Type | polyclonal |
Conjugation | Alexa Fluor 647 |
Description | Alexa Fluor® 647-conjugated AffiniPure Sheep Anti-Mouse IgG, F(ab')2 Fragment Specific (min X Hu,Bov,Hrs Sr Prot) |
Recommended Dilution | 1:100 - 1:800 for most applications Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically. |
Supplier | Jackson ImmunoResearch |
Format | Affinity Purified |
Immunogen | IgG, F(ab')2 fragment |
Isotype | IgG |
Notes | Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective. Based on immunoelectrophoresis and/or ELISA, the antibody reacts with the F(ab')2/Fab portion of mouse IgG. It also reacts with the light chains of other mouse immunoglobulins. No antibody was detected against the Fc portion of mouse IgG or against non-immunoglobulin serum proteins. The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with human, bovine and horse serum proteins, but it may cross-react with immunoglobulins from other species. |
Product Type | Antibodies Secondary |
Protocol | 1:100 - 1:800 for most applications Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically. |
Purity | AffiniPure |
Shipping Information | RT |
Size | 1 mg |
Source / Host | sheep/ovine |
Species Reactivity | mouse |
Storage | 4°C |
Substrate / Buffer | 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6 |
Product Page Updated | 2023-12-29T14:15:50.354Z |