Article No
712-685-150
Brilliant Violet™ 480-conjugated AffiniPure Donkey Anti-Rat IgG (H+L) (min X Bov,Ck,Gt,GP,Sy Hms,Hrs,Hu,Rb,Shp Sr Prot)
Brilliant Violet™ 480-conjugated AffiniPure Donkey Anti-Rat IgG (H+L) (min X Bov,Ck,Gt,GP,Sy Hms,Hrs,Hu,Rb,Shp Sr Prot)
Antibodies Secondary
Specifications
| Application | IF, FC |
| Article No | 712-685-150 |
| Country Availability | SE, FI, DK, NO, IS, EE, LV, LT |
| Clone Type | polyclonal |
| Conjugation | Brilliant Violet 480 |
| Description | Brilliant Violet™ 480-conjugated AffiniPure Donkey Anti-Rat IgG (H+L) (min X Bov,Ck,Gt,GP,Sy Hms,Hrs,Hu,Rb,Shp Sr Prot) |
| Recommended Dilution | _x000D_ 1:50 - 1:200 for most applications_x000D_ _x000D_ Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically._x000D_ |
| Supplier | Jackson ImmunoResearch |
| Format | Affinity Purified |
| Immunogen | IgG (H+L) |
| Isotype | IgG |
| Notes | Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective. Based on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule rat IgG. It also reacts with the light chains of other rat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with bovine, chicken, goat, guinea pig, syrian hamster, horse, human, rabbit and sheep serum proteins, but it may cross-react with immunoglobulins from other species. |
| Product Type | Antibodies Secondary |
| Protocol | 1:50 - 1:200 for most applications Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically. |
| Purity | AffiniPure |
| Shipping Information | RT |
| Size | 0.25 ml |
| Source / Host | donkey |
| Species Reactivity | rat |
| Storage | 4°C |
| Substrate / Buffer | 0.01M Sodium Phosphate, 0.15M NaCl, 0.005% Tween 20, pH 7.2 |
| Product Page Updated | 2023-12-29T14:15:50.354Z |
