Article No
715-055-140
Application | ELISA, WB |
Article No | 715-055-140 |
Country Availability | SE, FI, DK, NO, IS, EE, LV, LT |
Clone Type | polyclonal |
Conjugation | AP |
Description | Alkaline Phosphatase-conjugated AffiniPure Donkey Anti-Mouse IgM, µ Chain Specific (min X Hu,Bov,Hrs,Rat Sr Prot) |
Recommended Dilution | ELISA 1:5,000-1:50,000, WB 1:5,000-1:50,000 |
Supplier | Jackson ImmunoResearch |
Format | Affinity Purified |
Immunogen | IgM, µ chain |
Isotype | IgM |
Notes | Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective. |
Product Type | Antibodies Secondary |
Protocol | ELISA:- 1:5,000-1:50,000Western Blot:- 1:5,000-1:50,000Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically. |
Purity | AffiniPure |
Shipping Information | RT |
Size | 0.5 ml |
Source / Host | donkey |
Species Reactivity | mouse |
Storage | 4°C |
Substrate / Buffer | 0.01M Tris-HCl, 0.25M NaCl, pH 8.0 |
Product Page Updated | 2023-12-29T14:15:50.354Z |