Article No
715-606-020
Alexa Fluor® 647-conjugated AffiniPure F(ab')2 Fragment Donkey Anti-Mouse IgM, µ Chain Specific
Alexa Fluor® 647-conjugated AffiniPure F(ab')2 Fragment Donkey Anti-Mouse IgM, µ Chain Specific
Antibodies Secondary
Specifications
| Application | IF, FC |
| Article No | 715-606-020 |
| Country Availability | SE, FI, DK, NO, IS, EE, LV, LT |
| Clone Type | polyclonal |
| Conjugation | Alexa Fluor 647 |
| Description | Alexa Fluor® 647-conjugated AffiniPure F(ab')2 Fragment Donkey Anti-Mouse IgM, µ Chain Specific |
| Recommended Dilution | 1:100 - 1:800 for most applications Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically. |
| Supplier | Jackson ImmunoResearch |
| Format | Affinity Purified |
| Immunogen | IgM, µ chain |
| Isotype | IgM |
| Notes | F(ab')2 fragment antibodies are generated by pepsin digestion of whole IgG antibodies to remove most of the Fc region while leaving some of the hinge region. F(ab')2 fragments have two antigen-binding Fab portions linked together by disulfide bonds and therefore they are divalent. The average molecular weight is about 110 kDa. They are used for specific applications, such as to avoid binding of secondary antibodies to live cells with Fc receptors or to Protein A or Protein G. Based on immunoelectrophoresis and/or ELISA, the antibody reacts with the heavy chain of mouse IgM but not with mouse IgG or the light chains of mouse immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody may cross-react with IgM from other species. |
| Product Type | Antibodies Secondary |
| Protocol | 1:100 - 1:800 for most applications Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically. |
| Purity | AffiniPure |
| Shipping Information | RT |
| Size | 0.75 mg |
| Source / Host | donkey |
| Species Reactivity | mouse |
| Storage | 4°C |
| Substrate / Buffer | 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6 |
| Product Page Updated | 2023-12-29T14:15:50.354Z |
