Article No
851.660.015
Human CD106 ELISA Set
Human CD106 ELISA Set
Assay & Detection
Specifications
| Application | ELISA |
| Article No | 851.660.015 |
| Country Availability | SE, FI, DK, NO, IS, EE, LV, LT, FO, GL |
| Description | Human CD106 ELISA Set |
| Supplier | Diaclone |
| Notes | No cross reactivity with other human adhesion molecules. |
| Alias Names | VCAM-1, INCAM-110, VCAM1, VCAM 1, DKFZp779G2333, INCAM 100, L1CAM, MGC99561, Vascular Cell Adhesion Molecule 1 |
| Product Type | Assay & Detection |
| Protocol | Dilute your standards and samples in Standard Diluent Buffer or in the most relevant matrix to your samples (i.e. Serum, Cell-culture medium). For cell-culture medium we recommend the use of standard Diluent Buffer, because biologicals fluids might contain proteases or cytokine-binding proteins that could modify the recognition of the cytokine you want to measure. . Dispense 100μl of dilution in each well. You should include blank wells (Zero Standard concentration). Remember to run standard and samples in Duplicate. See plate scheme below. . Reconstitute the vial of biotinylated detection anti-VCAM-1 with 0.55 mL of PBS 0.1% Azide. For 1 plate dilute 100 μl of the reconstituted vial into 5 mL of Biotinylated Antibody Diluent Buffer and distribute 50 μl in each well. . Co-Incubate the antigen and the biotinylated detection antibody for 1 hour at room temperature. . Wash 3 times with approximately 400μl of wash buffer and move immediatly to the next step. |
| Research area | Immunology, Inflammation, Cell Signaling |
| Size | 15 x 96 |
| Species Reactivity | human |
| Storage | Store all Reagents at 4°C |
| Technical Specifications | Pre-titrated antibodies, standards, Strep-HRP and TMB |
| Product Page Updated | 2024-01-03T10:09:04.762Z |
