Lysates

293 Lysate

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293 Lysate

Article No

ACC-450271

Application

WB

Size

0.1 mg

Suggested protocols

Specifications

Application WB
Article No ACC-450271
Country Availability SE, FI, DK, NO, IS, EE, LV, LT
Description 293 Lysate
Supplier Abbiotec
Notes 293 lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 ug/ml of aprotinin, 5 ug/ml of leupeptin. Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The 293 lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% beta-mercaptoethanol.
Alias Names transformed primary embryonal kidney
Product Type Lysates
Protocol WB: Boil the mixture for 10 min before loading. Load 5-20 ug lysate per lane.
Size 0.1 mg
Storage Store at 2-8°C for continuous use. For extended storage, freeze working aliquots at -80°C. Minimize freeze-thaw cycles. Product is guaranteed 6 months from the date of shipment.
Technical Specifications Lysate is supplied at 2 mg/ml in 1X SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% beta-mercaptoethanol.
Product Page Updated 2018-05-11T08:38:01.619Z

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