Article No
ACC-450271
Suggested protocols
Specifications
| Application | WB |
| Article No | ACC-450271 |
| Country Availability | SE, FI, DK, NO, IS, EE, LV, LT |
| Description | 293 Lysate |
| Supplier | Abbiotec |
| Notes | 293 lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 ug/ml of aprotinin, 5 ug/ml of leupeptin. Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The 293 lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% beta-mercaptoethanol. |
| Alias Names | transformed primary embryonal kidney |
| Product Type | Lysates |
| Protocol | WB: Boil the mixture for 10 min before loading. Load 5-20 ug lysate per lane. |
| Size | 0.1 mg |
| Storage | Store at 2-8°C for continuous use. For extended storage, freeze working aliquots at -80°C. Minimize freeze-thaw cycles. Product is guaranteed 6 months from the date of shipment. |
| Technical Specifications | Lysate is supplied at 2 mg/ml in 1X SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% beta-mercaptoethanol. |
| Product Page Updated | 2018-05-11T08:38:01.619Z |
Shipping info
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