Products ({{ products.totalElements }})
News & articles ({{ wpNewsAndArticles.total_posts }})
Blog entries ({{ wpBlogEntries.total_posts }})

{{ String(product.attributes.name) }} {{ String(product.attributes.productType) }} - NEW	{{ String(product.attributes.artNo) }}	{{ (String(product.attributes.sizeStr) != 'undefined') ? String(product.attributes.sizeStr) : '–' }}	Not available On Request {{ formatPrice(product.attributes.discountPrice.toString()) }} {{ formatPrice(product.attributes.price.toString()) }} {{ formatPrice(product.attributes.discountPrice.toString()) }} {{ formatPrice(product.attributes.price.toString()) }} Login for price

Full search results →

No products found

News {{ news.date \| formatDate }} #{{tag.name}}

No news & articles found

Full search results →

blog entry {{ blogEntry.date \| formatDate }}

No blog entries found

Full search results →

Anti-Hu CD46 Purified Azide Free

Antibodies Primary

Make a customized order

Article No

AFC-VB6WHU-0.1

Size

0.1 mg

Clone

MEM-258

Source / Host

mouse

Application

FC, IP, WB

Species Reactivity

cow/bovine

human

Article No

AFC-VB6WHU-0.1

Size

0.1 mg

Clone

MEM-258

Source / Host

mouse

Application

FC, IP, WB

Species Reactivity

cow/bovine

human

Specifications
Suggested protocols
References

Specifications

Application	FC, IP, WB
Article No	AFC-VB6WHU-0.1
Biosite Brand	Biosite Flow
Country Availability	all
Clone	MEM-258
Clone Type	monoclonal
Concentration	1 mg/ml
Conjugation	Unconjugated
Description	Anti-Hu CD46 Purified Azide Free
Supplier	Nordic Biosite
Entrez Gene ID	4179
Format	Purified Azide Free
Immunogen	HPB-ALL human T cell line
Isotype	IgG1
Keywords	CD Antigens & MHC (Veterinary), CD and Related Antigens (Human)
Notes	CD46 (MCP, membrane cofactor protein) is a multifunctional cell surface transmembrane protein that binds and inactivates C3b and C4b complement fragments, regulates T cell-induced inflammatory responses by either inhibiting (CD46-1 isoform) or increasing (CD46-2 isoform) the contact hypersensitivity reaction. CD46 also serves as a receptor for several human pathogens (both bacteria and viruses), and its ligation alteres T lymphocyte polarization toward antigen-presenting cells or target cells, inhibiting lymphocyte function. CD46 is a protector of placental tissue and is also expressed on the inner acrosomal membrane of spermatozoa.
Alias Names	MCP, TLX, AHUS2, MIC10, MGC26544
Previous Article No	AFC-4092-2, AFC-VB6WHU-0.1
Product Type	Antibodies Primary
Protocol	Flow cytometry: Recommended dilution: 1 μg/ml. Western blotting: Non-reducing conditions.
Purification	Purified by protein-A affinity chromatography.
Purity	> 95% (by SDS-PAGE)
Research area	Immunology
Size	0.1 mg
Source / Host	mouse
Species Reactivity	cow/bovine, human
Storage	Store at 2-8°C. Do not freeze.
Substrate / Buffer	Phosphate buffered saline (PBS), pH 7.4
Technical Specifications	The antibody MEM-258 recognizes an extracellular epitope on SCR4 (the membrane-proximal SCR) domain of CD46 (Membrane cofactor protein). CD46 is 56-66 kDa dimeric transmembrane protein expressed on T and B lymphocytes, platelets, monocytes, granulocytes, endothelial cells, epithelial cells and fibroblast; it is negative on erythrocytes.
UniProt Number	P15529
Product Page Updated	2024-01-03T12:18:20.318Z

Suggested protocols

Flow Cytometry

Immunoprecipitation

Western Blot

References

*Kälin S, Amstutz B, Gastaldelli M, Wolfrum N, Boucke K, Havenga M, DiGennaro F, Liska N, Hemmi S, Greber UF: Macropinocytotic uptake and infection of human epithelial cells with species B2 adenovirus type 35. J Virol. 2010 May;84(10):5336-50., URL: http://www.ncbi.nlm.nih.gov/pubmed/20237079 ,*Rebetz J, Na M, Su C, Holmqvist B, Edqvist A, Nyberg C, Widegren B, Salford LG, Sjögren HO, Arnberg N, Qian Q, Fan X: Fiber mediated receptor masking in non-infected bystander cells restricts adenovirus cell killing effect but promotes adenovirus host co-existence. PLoS One. 2009 Dec 29;4(12):e8484., URL: http://www.ncbi.nlm.nih.gov/pubmed/20041185 ,*Wang H, Tuve S, Erdman DD, Lieber A: Receptor usage of a newly emergent adenovirus type 14. Virology. 2009 May 10;387(2):436-41., URL: http://www.ncbi.nlm.nih.gov/pubmed/19307010 ,*Hoffmann D, Bayer W, Heim A, Potthoff A, Nettelbeck DM, Wildner O: Evaluation of twenty-one human adenovirus types and one infectivity-enhanced adenovirus for the treatment of malignant melanoma. J Invest Dermatol. 2008 Apr;128(4):988-98., URL: http://www.ncbi.nlm.nih.gov/pubmed/17960177 ,*Fleischli C, Sirena D, Lesage G, Havenga MJ, Cattaneo R, Greber UF, Hemmi S: Species B adenovirus serotypes 3, 7, 11 and 35 share similar binding sites on the membrane cofactor protein CD46 receptor. J Gen Virol. 2007 Nov;88(Pt 11):2925-34., URL: http://www.ncbi.nlm.nih.gov/pubmed/17947513 ,*Loré K, Adams WC, Havenga MJ, Precopio ML, Holterman L, Goudsmit J, Koup RA: Myeloid and plasmacytoid dendritic cells are susceptible to recombinant adenovirus vectors and stimulate polyfunctional memory T cell responses. J Immunol. 2007 Aug 1;179(3):1721-9., URL: http://www.ncbi.nlm.nih.gov/pubmed/17641038 ,*Fremeaux-Bacchi V, Moulton EA, Kavanagh D, Dragon-Durey MA, Blouin J, Caudy A, Arzouk N, Cleper R, Francois M, Guest G, Pourrat J, Seligman R, Fridman WH, Loirat C, Atkinson JP: Genetic and functional analyses of membrane cofactor protein (CD46) mutations in atypical hemolytic uremic syndrome. J Am Soc Nephrol. 2006 Jul;17(7):2017-25., URL: http://www.ncbi.nlm.nih.gov/pubmed/16762990 ,*Weyand NJ, Lee SW, Higashi DL, Cawley D, Yoshihara P, So M: Monoclonal antibody detection of CD46 clustering beneath Neisseria gonorrhoeae microcolonies. Infect Immun. 2006 Apr;74(4):2428-35., URL: http://www.ncbi.nlm.nih.gov/pubmed/16552073 ,*Fleischli C, Verhaagh S, Havenga M, Sirena D, Schaffner W, Cattaneo R, Greber UF, Hemmi S: The distal short consensus repeats 1 and 2 of the membrane cofactor protein CD46 and their distance from the cell membrane determine productive entry of species B adenovirus serotype 35. J Virol. 2005 Aug;79(15):10013-22., URL: https://www.ncbi.nlm.nih.gov/pubmed/16014961,,*Okada N, Liszewski MK, Atkinson JP, Caparon M: Membrane cofactor protein (CD46) is a keratinocyte receptor for the M protein of the group A streptococcus. Proc Natl Acad Sci U S A. 1995 Mar 28;92(7):2489-93., URL: https://www.ncbi.nlm.nih.gov/pubmed/7708671?ordina,*Marie JC, Astier AL, Rivailler P, Rabourdin-Combe C, Wild TF, Horvat B: Linking innate and acquired immunity: divergent role of CD46 cytoplasmic domains in T cell induced inflammation. Nat Immunol. 2002 Jul;3(7):659-66., URL: https://www.ncbi.nlm.nih.gov/pubmed/12055630?ordin,*Gaggar A, Shayakhmetov DM, Lieber A: CD46 is a cellular receptor for group B adenoviruses. Nat Med. 2003 Nov;9(11):1408-12., URL: https://www.ncbi.nlm.nih.gov/pubmed/14566335?ordin,*Liszewski MK, Kemper C, Price JD, Atkinson JP: Emerging roles and new functions of CD46. Springer Semin Immunopathol. 2005 Nov;27(3):345-58., URL: https://www.ncbi.nlm.nih.gov/pubmed/16200405?ordin,*Oliaro J, Pasam A, Waterhouse NJ, Browne KA, Ludford-Menting MJ, Trapani JA, Russell SM: Ligation of the cell surface receptor, CD46, alters T cell polarity and response to antigen presentation. Proc Natl Acad Sci U S A. 2006 Dec 5;103(49):18685-90. , URL: https://www.ncbi.nlm.nih.gov/pubmed/17116876?ordin,,

Shipping info

The delivery time for this item is approximately 8-16 business days. Read more