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Anti-Hu CD56 Purified

Antibodies Primary

Make a customized order

Article No

AFC-ITPO87-0.1

Size

0.1 mg

Clone

MEM-188

Source / Host

mouse

Application

FC, IP, IHC, ICC

Species Reactivity

monkey

human

Article No

AFC-ITPO87-0.1

Size

0.1 mg

Clone

MEM-188

Source / Host

mouse

Application

FC, IP, IHC, ICC

Species Reactivity

monkey

human

Specifications
Suggested protocols
References

Specifications

Application	FC, IP, IHC, ICC
Article No	AFC-ITPO87-0.1
Biosite Brand	Biosite Flow
Country Availability	all
Clone	MEM-188
Clone Type	monoclonal
Concentration	1 mg/ml
Conjugation	Unconjugated
Description	Anti-Hu CD56 Purified
Supplier	Nordic Biosite
Entrez Gene ID	4684
Format	Purified
Immunogen	KG-1 human acute myelogenous leukemia cell line
Isotype	IgG2a
Keywords	CD Antigens & MHC (Veterinary), CD and Related Antigens (Human)
Notes	CD56 (NCAM, neural cell adhesion molecule) is a transmembrane glycoprotein of immunoglobulin family serving as adhesive molecule which is ubiquitously expressed in nervous system, usually as 120 kDa, 140 kDa or 180 kDa isoform, and it is also found on T cells and NK cells. Polysialic modification results in reduction of CD56-mediated cell adhesion and is involved in cell migration, axonal growth, pathfinding and synaptic plasticity. CD56 is a widely used neuroendocrine marker with a high sensitivity for neuroendocrine tumours and ovarian granulosa cell tumours.
Alias Names	NCAM1, MSK39
Previous Article No	AFC-4181-1, AFC-ITPO87-0.1
Product Type	Antibodies Primary
Protocol	Flow cytometry: Recommended dilution: 3 μg/ml. Immunohistochemistry (paraffin sections): Recommended dilution: 1:50; pretreatment: heat retrieval (microwave oven) in citrate buffer, standard ABC visualization; positive tissue: membrane-specific reaction in adjacent peripheral nerve; staining of paraprostatic autonomic ganglion.
Purification	Purified by protein-A affinity chromatography.
Purity	> 95% (by SDS-PAGE)
Research area	Immunology
Size	0.1 mg
Source / Host	mouse
Species Reactivity	monkey, human
Storage	Store at 2-8°C. Do not freeze.
Substrate / Buffer	Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Technical Specifications	The antibody MEM-188 reacts with an extracellular epitope on a 180 kDa isoform of CD56 (NCAM) expressed in leukocytes. It has been suggested that the antibody MEM-188 could react with rhesus monkey lymphocytes. Reactivity with other NCAM isoforms has not been tested.
UniProt Number	P13591
Product Page Updated	2024-01-03T12:18:20.318Z

Suggested protocols

Flow Cytometry

IHC

Immunoprecipitation

References

*Brdicka T, Imrich M, Angelisová P, Brdicková N, Horváth O, Spicka J, Hilgert I, Lusková P, Dráber P, Novák P, Engels N, Wienands J, Simeoni L, Osterreicher J, Aguado E, Malissen M, Schraven B, Horejsí V: Non-T cell activation linker (NTAL): a transmembrane adaptor protein involved in immunoreceptor signaling. J Exp Med. 2002 Dec 16;196(12):1617-26., URL: https://www.ncbi.nlm.nih.gov/pubmed/12486104?itool,*Olsen I, Tollefsen S, Aagaard C, Reitan LJ, Bannantine JP, Andersen P, Sollid LM, Lundin KE: Isolation of Mycobacterium avium subspecies paratuberculosis reactive CD4 T cells from intestinal biopsies of Crohn's disease patients., URL: https://www.ncbi.nlm.nih.gov/pubmed/19479064?itool,*Yates J, Rovis F, Mitchell P, Afzali B, Tsang JY, Garin M, Lechler RI, Lombardi G, Garden OA: The maintenance of human CD4+ CD25+ regulatory T cell function: IL-2, IL-4, IL-7 and IL-15 preserve optimal suppressive potency in vitro. Int Immunol. 2007 Jun;19(6):785-99., URL: http://www.ncbi.nlm.nih.gov/pubmed/17545278 ,*Hovden AO, Karlsen M, Jonsson R, Aarstad HJ, Appel S: Maturation of monocyte derived dendritic cells with OK432 boosts IL-12p70 secretion and conveys strong T-cell responses. BMC Immunol. 2011 Jan 5;12:2., URL: http://www.ncbi.nlm.nih.gov/pubmed/21208424 ,*Kanderova V, Kuzilkova D, Stuchly J, Vaskova M, Brdicka T, Fiser K, Hrusak O, Lund-Johansen F, Kalina T: High-resolution Antibody Array Analysis of Childhood Acute Leukemia Cells. Mol Cell Proteomics. 2016 Apr;15(4):1246-61., URL: http://www.ncbi.nlm.nih.gov/pubmed/26785729 ,*Leukocyte Typing VI., Kishimoto T. et al. (Eds.), Garland Publishing Inc. (1997)., URL: ,*Leukocyte Typing VII., Mason D. et al. (Eds.), Oxford University Press (2002)., URL: ,*Lin CW, Liu TY, Chen SU, Wang KT, Medeiros LJ, Hsu SM: CD94 1A transcripts characterize lymphoblastic lymphoma/leukemia of immature natural killer cell origin with distinct clinical features. Blood. 2005 Nov 15;106(10):3567-74., URL: https://www.ncbi.nlm.nih.gov/pubmed/16046525?itool,*Brdicková N, Brdicka T, Angelisová P, Horváth O, Spicka J, Hilgert I, Paces J, Simeoni L, Kliche S, Merten C, Schraven B, Horejsí V: LIME: a new membrane Raft-associated adaptor protein involved in CD4 and CD8 coreceptor signaling. J Exp Med. 2003 Nov 17;198(10):1453-62., URL: https://www.ncbi.nlm.nih.gov/pubmed/14610046?itool,*Drbal K, Moertelmaier M, Holzhauser C, Muhammad A, Fuertbauer E, Howorka S, Hinterberger M, Stockinger H, Schütz GJ: Single-molecule microscopy reveals heterogeneous dynamics of lipid raft components upon TCR engagement. Int Immunol. 2007 May;19(5):675-84., URL: https://www.ncbi.nlm.nih.gov/pubmed/17446208?itool,,*McCluggage WG, McKenna M, McBride HA: CD56 is a sensitive and diagnostically useful immunohistochemical marker of ovarian sex cord-stromal tumors. Int J Gynecol Pathol. 2007 Jul;26(3):322-7., URL: https://www.ncbi.nlm.nih.gov/pubmed/17581419?ordin,*Ohishi Y, Kaku T, Oya M, Kobayashi H, Wake N, Tsuneyoshi M: CD56 expression in ovarian granulosa cell tumors, and its diagnostic utility and pitfalls. Gynecol Oncol. 2007 Oct;107(1):30-8., URL: https://www.ncbi.nlm.nih.gov/pubmed/17583777?ordin,*Jakovcevski I, Mo Z, Zecevic N: Down-regulation of the axonal polysialic acid-neural cell adhesion molecule expression coincides with the onset of myelination in the human fetal forebrain. Neuroscience. 2007 Oct 26;149(2):328-37., URL: https://www.ncbi.nlm.nih.gov/pubmed/17900814?ordin,*Lanier LL, Chang C, Azuma M, Ruitenberg JJ, Hemperly JJ, Phillips JH: Molecular and functional analysis of human natural killer cell-associated neural cell adhesion molecule (N-CAM/CD56). J Immunol. 1991 Jun 15;146(12):4421-6., URL: https://www.ncbi.nlm.nih.gov/pubmed/1710251?ordina,,

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