Article No
AFC-Y0SALM-0.1
Application | FC, WB, IHC, ICC |
Article No | AFC-Y0SALM-0.1 |
Biosite Brand | Biosite Flow |
Country Availability | all |
Clone | MEM-05 |
Clone Type | monoclonal |
Concentration | 1 mg/ml |
Conjugation | Unconjugated |
Description | Anti-Hu CD31 Purified |
Supplier | Nordic Biosite |
Entrez Gene ID | 5175 |
Format | Purified |
Immunogen | Leukocytes of a patient suffering from LGL-type leukaemia |
Isotype | IgG1 |
Keywords | CD and Related Antigens (Human) |
Notes | CD31 (platelet endothelial cell adhesion molecule-1, PECAM-1) is an inhibitory coreceptor involved in regulation of T cell and B cell signaling by a dual immunoreceptor tyrosine-based inhibitory motif (ITIM) that upon associated kinases-mediated phosphorylation provide docking sites for protein-tyrosine phosphatases. CD31 is expressed ubiquitously within the vascular compartment and is located mainly at junctions between adjacent cells. N-terminal Ig-like domain of CD31 is responsible for its homophilic binding, which plays an important role in cell-cell interactions. CD31 is a multifunctional molecule with diverse roles in modulation of integrin-mediated cell adhesion, transendothelial migration, angiogenesis, apoptosis, negative regulation of immunoreceptor signaling, autoimmunity, macrophage phagocytosis, IgE-mediated anaphylaxis and thrombosis. It is one of key regulatory molecules in vascular system. |
Alias Names | PECAM-1, EndoCAM, GPIIA, PECA1 |
Previous Article No | AFC-4216-2, AFC-Y0SALM-0.1 |
Product Type | Antibodies Primary |
Protocol | Flow cytometry: Recommended dilution: 1-4 μg/ml. Western blotting: Non-reducing conditions. |
Purification | Purified by protein-A affinity chromatography. |
Purity | > 95% (by SDS-PAGE) |
Research Area | Immunology |
Size | 0.1 mg |
Source / Host | mouse |
Species Reactivity | human |
Storage | Store at 2-8°C. Do not freeze. |
Substrate / Buffer | Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
Technical Specifications | The antibody MEM-05 reacts with an extracellular epitope of CD31 (PECAM-1), a 130-140 kDa type I transmembrane glycoprotein expressed on monocytes, platelets, granulocytes, endothelial cells and stem cells of the myeloid lineage. |
UniProt Number | P16284 |
Product Page Updated | 2024-01-03T12:18:20.318Z |