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Anti-Hu CD106 Purified

Antibodies Primary

Make a customized order

Article No

AFC-GA76GW-0.1

Species Reactivity

human

Size

0.1 mg

Clone

STA

Source / Host

mouse

Application

FC, IP, IHC, ELISA

Article No

AFC-GA76GW-0.1

Species Reactivity

human

Size

0.1 mg

Clone

STA

Source / Host

mouse

Application

FC, IP, IHC, ELISA

Specifications
Suggested protocols
References

Specifications

Application	FC, IP, IHC, ELISA
Article No	AFC-GA76GW-0.1
Biosite Brand	Biosite Flow
Country Availability	all
Clone	STA
Clone Type	monoclonal
Concentration	1 mg/ml
Conjugation	Unconjugated
Description	Anti-Hu CD106 Purified
Supplier	Nordic Biosite
Entrez Gene ID	7412
Format	Purified
Immunogen	Human DS6 T cell line
Isotype	IgG1
Keywords	CD and Related Antigens (Human)
Notes	CD106 / VCAM-1 (vascular cell adhesion molecule-1) is an Ig-like cell surface adhesion molecule binding VLA-4 integrin. VCAM-1 is a potent T cell costimulatory molecule taking part in their positive selection and survival, as well as in adhesion, transendothelial migration and activation of peripheral T cells. VCAM-1 is also involved in endothelial cell-cell contacts. Whereas VCAM-1 normally mediates leukocyte extravasion to sites of tissue inflammation, tumour cells can use overexpressed VCAM-1 to escape T cell immunity. Soluble form of VCAM-1 (sVCAM-1) is an inflammatory marker and can be used also in prognosis of subsequent cariovascular events following acute coronary syndromes.
Alias Names	VCAM-1, INCAM-100
Previous Article No	AFC-4471-2, AFC-GA76GW-0.1
Product Type	Antibodies Primary
Protocol	Flow cytometry: Recommended dilution: 4-6 μg/ml; positive control: TNF-alpha activated HUVEC cells. Immunohistochemistry (frozen sections): Acetone fixation. ELISA: Capture mAb for soluble CD106.
Purification	Purified by protein-A affinity chromatography.
Purity	> 95% (by SDS-PAGE)
Research area	Immunology
Size	0.1 mg
Source / Host	mouse
Species Reactivity	human
Storage	Store at 2-8°C. Do not freeze.
Substrate / Buffer	Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Technical Specifications	The mouse monoclonal antibody STA recognizes an extracellular epitope of CD106 antigen (VCAM-1), a 100-110 kDa type I membrane protein of the immunoglobulin superfamily, a crucial mediator of leukocyte adhesion, and a costimulation molecule.
UniProt Number	P19320
Product Page Updated	2024-01-03T12:18:20.318Z

Suggested protocols

ELISA

Flow Cytometry

IHC

Immunoprecipitation

References

*Leukocyte Typing V., Schlossman S. et al. (Eds.), Oxford University Press (1995)., URL: ,*Leca G, Mansur SE, Bensussan A: Expression of VCAM-1 (CD106) by a subset of TCR gamma delta-bearing lymphocyte clones. Involvement of a metalloprotease in the specific hydrolytic release of the soluble isoform. J Immunol. 1995 Feb 1;154(3):1069-77., URL: https://www.ncbi.nlm.nih.gov/pubmed/7529789?ordina,*Yen YT, Liao F, Hsiao CH, Kao CL, Chen YC, Wu-Hsieh BA: Modeling the early events of severe acute respiratory syndrome coronavirus infection in vitro. J Virol. 2006 Mar;80(6):2684-93., URL: https://www.ncbi.nlm.nih.gov/pubmed/16501078?ordin,,*van Wetering S, van den Berk N, van Buul JD, Mul FP, Lommerse I, Mous R, ten Klooster JP, Zwaginga JJ, Hordijk PL: VCAM-1-mediated Rac signaling controls endothelial cell-cell contacts and leukocyte transmigration. Am J Physiol Cell Physiol. 2003 Aug;285(2):C343-52., URL: https://pubmed.ncbi.nlm.nih.gov/12700137/,*Wu TC: The role of vascular cell adhesion molecule-1 in tumor immune evasion. Cancer Res. 2007 Jul 1;67(13):6003-6., URL: https://pubmed.ncbi.nlm.nih.gov/17616653/,*Postadzhiyan AS, Tzontcheva AV, Kehayov I, Finkov B: Circulating soluble adhesion molecules ICAM-1 and VCAM-1 and their association with clinical outcome, troponin T and C-reactive protein in patients with acute coronary syndromes. Clin Biochem. 2007 Sep 19, URL: https://pubmed.ncbi.nlm.nih.gov/18061588/,*Paessens LC, Singh SK, Fernandes RJ, van Kooyk Y: Vascular cell adhesion molecule-1 (VCAM-1) and intercellular adhesion molecule-1 (ICAM-1) provide co-stimulation in positive selection along with survival of selected thymocytes. Mol Immunol. 2008 Jan;45(1):42-8., URL: https://pubmed.ncbi.nlm.nih.gov/17604837/,,

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