Article No
AFC-AFQ6WK-0.1
Anti-Hu CD3 zeta (pY111) Purified
Anti-Hu CD3 zeta (pY111) Purified
Antibodies Primary
Specifications
| Application | FC, WB |
| Article No | AFC-AFQ6WK-0.1 |
| Biosite Brand | Biosite Flow |
| Country Availability | all |
| Clone | EM-55 |
| Clone Type | monoclonal |
| Concentration | 1 mg/ml |
| Conjugation | Unconjugated |
| Description | Anti-Hu CD3 zeta (pY111) Purified |
| Supplier | Nordic Biosite |
| Entrez Gene ID | 919 |
| Format | Purified |
| Immunogen | BP1 (pTyr111/123) - KLH |
| Isotype | IgG1 |
| Keywords | CD and Related Antigens & MHC (Rodent), CD and Related Antigens (Human) |
| Notes | CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta (CD247). These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. |
| Alias Names | CD3 zeta chain, CD247, T3Z, TCRz, IMD25 |
| Previous Article No | AFC-4576-2, AFC-AFQ6WK-0.1 |
| Product Type | Antibodies Primary |
| Protocol | Flow cytometry: Recommended dilution: 1-4 μg/ml. Intracellular staining. |
| Purification | Purified by protein-A affinity chromatography. |
| Purity | > 95% (by SDS-PAGE) |
| Research area | Immunology |
| Size | 0.1 mg |
| Source / Host | mouse |
| Species Reactivity | mouse, human |
| Storage | Store at 2-8°C. Do not freeze. |
| Substrate / Buffer | Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
| Technical Specifications | The mouse monoclonal antibody EM-55 recognizes phosphorylated intracellular tyrosine 111 of CD3 zeta chain (CD247), which is a component of TCR/CD3 complex expressed on T cells. |
| UniProt Number | P20963 |
| Product Page Updated | 2024-01-03T12:18:20.318Z |
