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Anti-Hu CD62L Purified Low Endotoxin

Antibodies Primary

Make a customized order

Article No

AFC-B6YVAQ-0.1

Species Reactivity

human

Size

0.1 mg

Clone

DREG56

Source / Host

mouse

Application

FC, IP, WB, IHC

Article No

AFC-B6YVAQ-0.1

Species Reactivity

human

Size

0.1 mg

Clone

DREG56

Source / Host

mouse

Application

FC, IP, WB, IHC

Specifications
Suggested protocols
References

Specifications

Application	FC, IP, WB, IHC
Article No	AFC-B6YVAQ-0.1
Biosite Brand	Biosite Flow
Country Availability	all
Clone	DREG56
Clone Type	monoclonal
Concentration	1 mg/ml
Conjugation	Unconjugated
Description	Anti-Hu CD62L Purified Low Endotoxin
Supplier	Nordic Biosite
Entrez Gene ID	6402
Format	Purified Low Endotoxin
Immunogen	PMA-activated human peripheral blood leukocytes
Isotype	IgG1
Keywords	CD and Related Antigens (Human)
Notes	CD62L (L-selectin) is an adhesion glycoprotein that is constitutively expressed on the cell surface of leukocytes and mediates their homing to inflammatory sites and peripheral lymph nodes by enabling rolling along the venular wall. CD62L is also involved in activation-induced neutrophil aggregation. Activation-dependent CD62L shedding, however, counteracts neutrophil rolling. CD62L has also signaling roles including enhance of chemokine receptor expression. Similarly to CD62P, the major ligand of CD62L is PSGL-1 (P-selectin glycoprotein ligand-1).
Alias Names	L-selectin, LSEL, SELL, LAM-1, LECAM1, LEU8, LNHR, LYAM1, PLNHR
Previous Article No	AFC-4657-2, AFC-B6YVAQ-0.1
Product Type	Antibodies Primary
Protocol	Functional application: Particularly effective in blocking of lymphocyte CD62L-mediated binding to peripheral lymph node HEV. Flow cytometry: Recommended dilution: 2 μg/ml. Western blotting: Non-reducing conditions.
Purification	Purified by protein-A affinity chromatography.
Purity	> 95% (by SDS-PAGE)
Research area	Immunology
Size	0.1 mg
Source / Host	mouse
Species Reactivity	human
Storage	Store at 2-8°C. Do not freeze.
Substrate / Buffer	Phosphate buffered saline (PBS), pH 7.4
Technical Specifications	The mouse monoclonal antibody DREG56 recognizes an extracellular epitope of CD62L / L-selectin, a 65-76 kDa cell surface protein, expressed by neutrophils, monocytes, and subsets of T, B, and NK cells, that interacts with specific carbohydrates exposed on activated endothelial cells.
UniProt Number	P14151
Product Page Updated	2024-01-03T12:18:20.318Z

Suggested protocols

Flow Cytometry

IHC

Immunoprecipitation

Western Blot

References

*Abraham WM, Ahmed A, Sabater JR, Lauredo IT, Botvinnikova Y, Bjercke RJ, Hu X, Revelle BM, Kogan TP, Scott IL, Dixon RA, Yeh ET, Beck PJ: Selectin blockade prevents antigen-induced late bronchial responses and airway hyperresponsiveness in allergic sheep. Am J Respir Crit Care Med. 1999 Apr;159(4 Pt 1):1205-14., URL: https://www.ncbi.nlm.nih.gov/pubmed/10194167,*Xu T, Chen L, Shang X, Cui L, Luo J, Chen C, Ba X, Zeng X: Critical role of Lck in L-selectin signaling induced by sulfatides engagement. J Leukoc Biol. 2008 Oct;84(4):1192-201., URL: https://www.ncbi.nlm.nih.gov/pubmed/18653462,*Killcock DJ, Parsons M, Zarrouk M, Ameer-Beg SM, Ridley AJ, Haskard DO, Zvelebil M, Ivetic A: In Vitro and in Vivo Characterization of Molecular Interactions between Calmodulin, Ezrin/Radixin/Moesin, and L-selectin. J Biol Chem. 2009 Mar 27;284(13):8833-45., URL: https://www.ncbi.nlm.nih.gov/pubmed/19129194,*Tu W, Mao H, Zheng J, Liu Y, Chiu SS, Qin G, Chan PL, Lam KT, Guan J, Zhang L, Guan Y, Yuen KY, Peiris JS, Lau YL: Cytotoxic T lymphocytes established by seasonal human influenza cross-react against 2009 pandemic H1N1 influenza virus. J Virol. 2010 Jul;84(13):6527-35., URL: http://www.ncbi.nlm.nih.gov/pubmed/20410263 ,*Leukocyte Typing V., Schlossman S. et al. (Eds.), Oxford University Press (1995)., URL: ,*Leukocyte Typing VI., Kishimoto T. et al. (Eds.), Garland Publishing Inc. (1997)., URL: ,*Jutila MA, Kurk S, Jackiw L, Knibbs RN, Stoolman LM: L-selectin serves as an E-selectin ligand on cultured human T lymphoblasts. J Immunol. 2002 Aug 15;169(4):1768-73., URL: https://www.ncbi.nlm.nih.gov/pubmed/12165498,*Kishimoto TK, Jutila MA, Butcher EC: Identification of a human peripheral lymph node homing receptor: a rapidly down-regulated adhesion molecule. Proc Natl Acad Sci U S A. 1990 Mar;87(6):2244-8., URL: https://www.ncbi.nlm.nih.gov/pubmed/2179952,*Kishimoto TK, Warnock RA, Jutila MA, Butcher EC, Lane C, Anderson DC, Smith CW: Antibodies against human neutrophil LECAM-1 (LAM-1/Leu-8/DREG-56 antigen) and endothelial cell ELAM-1 inhibit a common CD18-independent adhesion pathway in vitro. Blood. 1991 Aug 1;78(3):805-11., URL: https://www.ncbi.nlm.nih.gov/pubmed/1713515,,*Ramachandran V, Williams M, Yago T, Schmidtke DW, McEver RP: Dynamic alterations of membrane tethers stabilize leukocyte rolling on P-selectin. Proc Natl Acad Sci U S A. 2004 Sep 14;101(37):13519-24., URL: https://www.ncbi.nlm.nih.gov/pubmed/15353601?ordin,*Ding Z, Issekutz TB, Downey GP, Waddell TK: L-selectin stimulation enhances functional expression of surface CXCR4 in lymphocytes: implications for cellular activation during adhesion and migration. Blood. 2003 Jun 1;101(11):4245-52., URL: https://www.ncbi.nlm.nih.gov/pubmed/12609846?ordin,*Simon SI, Burns AR, Taylor AD, Gopalan PK, Lynam EB, Sklar LA, Smith CW: L-selectin (CD62L) cross-linking signals neutrophil adhesive functions via the Mac-1 (CD11b/CD18) beta 2-integrin. J Immunol. 1995 Aug 1;155(3):1502-14., URL: https://www.ncbi.nlm.nih.gov/pubmed/7543524?ordina,*von Andrian UH, Chambers JD, Berg EL, Michie SA, Brown DA, Karolak D, Ramezani L, Berger EM, Arfors KE, Butcher EC. L-selectin mediates neutrophil rolling in inflamed venules through sialyl LewisX-dependent and -independent recognition pathways. Blood. 1993 Jul 1;82(1):182-91., URL: https://www.ncbi.nlm.nih.gov/pubmed/7686786?ordina,*Von Andrian UH, Hansell P, Chambers JD, Berger EM, Torres Filho I, Butcher EC, Arfors KE: L-selectin function is required for beta 2-integrin-mediated neutrophil adhesion at physiological shear rates in vivo. Am J Physiol. 1992 Oct;263(4 Pt 2):H1034-44., URL: https://www.ncbi.nlm.nih.gov/pubmed/1384360?ordina,,

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