Products ({{ products.totalElements }})
News & articles ({{ wpNewsAndArticles.total_posts }})
Blog entries ({{ wpBlogEntries.total_posts }})

{{ String(product.attributes.name) }} {{ String(product.attributes.productType) }} - NEW	{{ String(product.attributes.artNo) }}	{{ (String(product.attributes.sizeStr) != 'undefined') ? String(product.attributes.sizeStr) : '–' }}	Not available On Request {{ formatPrice(product.attributes.discountPrice.toString()) }} {{ formatPrice(product.attributes.price.toString()) }} {{ formatPrice(product.attributes.discountPrice.toString()) }} {{ formatPrice(product.attributes.price.toString()) }} Login for price

Full search results →

No products found

News {{ news.date \| formatDate }} #{{tag.name}}

No news & articles found

Full search results →

blog entry {{ blogEntry.date \| formatDate }}

No blog entries found

Full search results →

Anti-Hu CD11b Purified Low Endotoxin

Antibodies Primary

Make a customized order

Article No

AFC-9CVADV-0.1

Size

0.1 mg

Clone

ICRF44

Source / Host

mouse

Application

FC, IP, IHC, ICC

Species Reactivity

human

monkey

Article No

AFC-9CVADV-0.1

Size

0.1 mg

Clone

ICRF44

Source / Host

mouse

Application

FC, IP, IHC, ICC

Species Reactivity

human

monkey

Specifications
Suggested protocols
References

Specifications

Application	FC, IP, IHC, ICC
Article No	AFC-9CVADV-0.1
Biosite Brand	Biosite Flow
Country Availability	all
Clone	ICRF44
Clone Type	monoclonal
Concentration	1 mg/ml
Conjugation	Unconjugated
Description	Anti-Hu CD11b Purified Low Endotoxin
Supplier	Nordic Biosite
Entrez Gene ID	3684
Format	Purified Low Endotoxin
Immunogen	Rheumatoid synovial cells and human monocytes.
Isotype	IgG1
Keywords	CD and Related Antigens (Human)
Notes	CD11b (integrin alphaM subunit) is a 165-170 kDa type I transmembrane glycoprotein that non-covalently associates with integrin beta2 subunit (CD18); expression of the CD11b chain on the cell surface requires the presence of the CD18 antigen. CD11b/CD18 integrin (Mac-1, CR3) is highly expressed on NK cells, neutrophils, monocytes and less on macrophages. CD11b/CD18 integrin is implicated in various adhesive interactions of monocytes, macrophages and granulocytes, facilitating their diapedesis, as well as it mediates the uptake of complement coated particles, serving as a receptor for the iC3b fragment of the third complement component.
Alias Names	Mac-1, Integrin alpha M, ITGAM, CR3A, MO1A, MAC1A
Previous Article No	AFC-4670-2, AFC-9CVADV-0.1
Product Type	Antibodies Primary
Protocol	Functional application: Blocking of granulocyte activation. Flow cytometry: Recommended dilution: 1-12 µg/ml
Purification	Purified by protein-A affinity chromatography.
Purity	> 95% (by SDS-PAGE)
Research area	Immunology
Size	0.1 mg
Source / Host	mouse
Species Reactivity	human, monkey
Storage	Store at 2-8°C. Do not freeze.
Substrate / Buffer	Phosphate buffered saline (PBS), pH 7.4
Technical Specifications	The mouse monoclonal antibody ICRF44 recognizes an extracellular epitope of CD11b (Mac-1alpha), a 165-170 kDa type 1 transmembrane protein mainly expressed on monocytes, granulocytes and NK-cells.
UniProt Number	P11215
Product Page Updated	2024-01-03T12:18:20.318Z

Suggested protocols

Flow Cytometry

IHC

Immunoprecipitation

References

*Machacek C, Supper V, Leksa V, Mitulovic G, Spittler A, Drbal K, Suchanek M, Ohradanova-Repic A, Stockinger H: Folate Receptor β Regulates Integrin CD11b/CD18 Adhesion of a Macrophage Subset to Collagen. J Immunol. 2016 Sep 15;197(6):2229-38., URL: https://www.ncbi.nlm.nih.gov/pubmed/27534550 ,*Hua J, Sakamoto K, Nagaoka I: Inhibitory actions of glucosamine, a therapeutic agent for osteoarthritis, on the functions of neutrophils. J Leukoc Biol. 2002 Apr;71(4):632-40., URL: https://www.ncbi.nlm.nih.gov/pubmed/11927650,*Sánchez-Torres C, García-Romo GS, Cornejo-Cortés MA, Rivas-Carvalho A, Sánchez-Schmitz G: CD16+ and CD16- human blood monocyte subsets differentiate in vitro to dendritic cells with different abilities to stimulate CD4+ T cells. Int Immunol. 2001 Dec;13(12):1571-81., URL: https://www.ncbi.nlm.nih.gov/pubmed/11717198,*Masten BJ, Olson GK, Tarleton CA, Rund C, Schuyler M, Mehran R, Archibeque T, Lipscomb MF: Characterization of myeloid and plasmacytoid dendritic cells in human lung. J Immunol. 2006 Dec 1;177(11):7784-93., URL: https://www.ncbi.nlm.nih.gov/pubmed/17114449,*Tieu BC, Lee C, Sun H, Lejeune W, Recinos A 3rd, Ju X, Spratt H, Guo DC, Milewicz D, Tilton RG, Brasier AR: An adventitial IL-6/MCP1 amplification loop accelerates macrophage-mediated vascular inflammation leading to aortic dissection in mice. J Clin Invest. 2009 Dec;119(12):3637-51., URL: https://www.ncbi.nlm.nih.gov/pubmed/19920349,*Rezzonico R, Imbert V, Chicheportiche R, Dayer JM: Ligation of CD11b and CD11c beta(2) integrins by antibodies or soluble CD23 induces macrophage inflammatory protein 1alpha (MIP-1alpha) and MIP-1beta production in primary human monocytes through a pathway dependent on nuclear factor-kappaB. Blood. 2001 May 15;97(10):2932-40., URL: https://www.ncbi.nlm.nih.gov/pubmed/11342414,,*Hentzen ER, Neelamegham S, Kansas GS, Benanti JA, McIntire LV, Smith CW, Simon SI. Sequential binding of CD11a/CD18 and CD11b/CD18 defines neutrophil capture and stable adhesion to intercellular adhesion molecule-1. Blood. 2000 Feb 1;95(3):911-20., URL: https://pubmed.ncbi.nlm.nih.gov/10648403/,*Lawrence PK, Srikumaran S: CD11b of Ovis canadensis and Ovis aries: molecular cloning and characterization. Vet Immunol Immunopathol. 2007 Oct 15;119(3-4):287-98., URL: https://pubmed.ncbi.nlm.nih.gov/17628696/,*Akramiene D, Kondrotas A, Didziapetriene J, Kevelaitis E: Effects of beta-glucans on the immune system. Medicina (Kaunas). 2007;43(8):597-606., URL: https://pubmed.ncbi.nlm.nih.gov/17895634/,,

Shipping info

The delivery time for this item is approximately 8-16 business days. Read more