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Anti-Hu CD62P APC

Antibodies Primary

Make a customized order

Article No

AFC-UI61N7-100

Application

Size

100 tests

Clone

AK4

Source / Host

mouse

Species Reactivity

human

monkey

Article No

AFC-UI61N7-100

Application

Size

100 tests

Clone

AK4

Source / Host

mouse

Species Reactivity

human

monkey

Specifications
Suggested protocols
References

Specifications

Application	FC
Article No	AFC-UI61N7-100
Biosite Brand	Biosite Flow
Country Availability	all
Clone	AK4
Clone Type	monoclonal
Conjugation	APC
Description	Anti-Hu CD62P APC
Recommended Dilution	The purified antibody is conjugated with cross-linked Allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.
Supplier	Nordic Biosite
Entrez Gene ID	6403
Format	APC
Immunogen	Human platelets
Isotype	IgG1 k
Keywords	CD Antigens & MHC (Veterinary), CD and Related Antigens (Human)
Notes	CD62P (P-selectin) is an adhesion glycoprotein that is expressed on platelets and endothelial cells upon their activation. Interaction between CD62P and its mucin-like ligand PSGL-1 (P-selectin glycoprotein ligand-1) expressed on the microvilli of most leukocytes supports leukocyte rolling along postkapillary venules at the earliest time of inflammation. Both CD62P and PSGL-1 are extended glycoproteins that form homodimers. CD62P dimerization is probably mediated through interactions of the transmembrane domains and stabilizes leukocyte tethering and rolling, probably by increasing rebinding within a bond cluster.
Alias Names	P-selectin, GMP140, SELP, PADGEM, GRMP, LECAM3, PSEL
Previous Article No	AFC-4804-2
Product Type	Antibodies Primary
Protocol	Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Purification	Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Research area	Immunology
Size	100 tests
Source / Host	mouse
Species Reactivity	human, monkey
Storage	Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Substrate / Buffer	Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Technical Specifications	The antibody AK4 recognizes an extracellular epitope of CD62P (P-selectin), a 140 kD single chain type I transmembrane glycoprotein present in secretory alpha-granules in platelets, in Weibel-Palade bodies in endothelial cells and in megakaryocytes; it is relocated to the plasma membrane upon activation.
UniProt Number	P16109
Product Page Updated	2024-01-03T12:18:20.318Z

Suggested protocols

Flow Cytometry

References

*Skinner MP, Lucas CM, Burns GF, Chesterman CN, Berndt MC: GMP-140 binding to neutrophils is inhibited by sulfated glycans. J Biol Chem. 1991 Mar 25;266(9):5371-4., URL: http://www.ncbi.nlm.nih.gov/pubmed/?term=skinner+mp+lucas+cm+burns+gf ,*Ludwig RJ, Schultz JE, Boehncke WH, Podda M, Tandi C, Krombach F, Baatz H, Kaufmann R, von Andrian UH, Zollner TM: Activated, not resting, platelets increase leukocyte rolling in murine skin utilizing a distinct set of adhesion molecules. J Invest Dermatol. 2004 Mar;122(3):830-6., URL: http://www.ncbi.nlm.nih.gov/pubmed/15086572 ,*Kowalska MA, Ratajczak J, Hoxie J, Brass LF, Gewirtz A, Poncz M, Ratajczak MZ: Megakaryocyte precursors, megakaryocytes and platelets express the HIV co-receptor CXCR4 on their surface: determination of response to stromal-derived factor-1 by megakaryocytes and platelets. Br J Haematol. 1999 Feb;104(2):220-9., URL: https://www.ncbi.nlm.nih.gov/pubmed/10050701,*Holme PA, Müller F, Solum NO, Brosstad F, Frøland SS, Aukrust P: Enhanced activation of platelets with abnormal release of RANTES in human immunodeficiency virus type 1 infection. FASEB J. 1998 Jan;12(1):79-89., URL: https://www.ncbi.nlm.nih.gov/pubmed/9438413,*Dunlop LC, Skinner MP, Bendall LJ, Favaloro EJ, Castaldi PA, Gorman JJ, Gamble JR, Vadas MA, Berndt MC: Characterization of GMP-140 (P-selectin) as a circulating plasma protein. J Exp Med. 1992 Apr 1;175(4):1147-50., URL: https://www.ncbi.nlm.nih.gov/pubmed/1372646,*Skinner MP, Lucas CM, Burns GF, Chesterman CN, Berndt MC: GMP-140 binding to neutrophils is inhibited by sulfated glycans. J Biol Chem. 1991 Mar 25;266(9):5371-4., URL: http://www.ncbi.nlm.nih.gov/pubmed/?term=skinner+mp+lucas+cm+burns+gf ,,*Harakawa N, Shigeta A, Wato M, Merrill-Skoloff G, Furie BC, Furie B, Okazaki T, Domae N, Miyasaka M, Hirata T. P-selectin glycoprotein ligand-1 mediates L-selectin-independent leukocyte rolling in high endothelial venules of peripheral lymph nodes. Int Immunol. 2007 Mar;19(3):321-9., URL: https://www.ncbi.nlm.nih.gov/pubmed/17267415?ordin,*Martinez M, Joffraud M, Giraud S, Baïsse B, Bernimoulin MP, Schapira M, Spertini O: Regulation of PSGL-1 interactions with L-selectin, P-selectin, and E-selectin: role of human fucosyltransferase-IV and -VII. J Biol Chem. 2005 Feb 18;280(7):5378-90., URL: https://www.ncbi.nlm.nih.gov/pubmed/15579466?ordin,*Ramachandran V, Williams M, Yago T, Schmidtke DW, McEver RP: Dynamic alterations of membrane tethers stabilize leukocyte rolling on P-selectin. Proc Natl Acad Sci U S A. 2004 Sep 14;101(37):13519-24., URL: https://www.ncbi.nlm.nih.gov/pubmed/15353601?ordin,*Ramachandran V, Yago T, Epperson TK, Kobzdej MM, Nollert MU, Cummings RD, Zhu C, McEver RP: Dimerization of a selectin and its ligand stabilizes cell rolling and enhances tether strength in shear flow. Proc Natl Acad Sci U S A. 2001 Aug 28;98(18):10166-71., URL: https://www.ncbi.nlm.nih.gov/pubmed/11481445?ordin,,

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