Article No
AFC-HHLOJ1-100
Specifications
| Application | FC |
| Article No | AFC-HHLOJ1-100 |
| Biosite Brand | Biosite Flow |
| Country Availability | all |
| Clone | S5/1 |
| Clone Type | monoclonal |
| Conjugation | PE |
| Description | Anti-Hu CD88 PE |
| Recommended Dilution | The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary. |
| Supplier | Nordic Biosite |
| Entrez Gene ID | 728 |
| Format | PE |
| Immunogen | Recombinant N-terminal peptide (Asp15-Asp27) of human C5aR |
| Isotype | IgG2a k |
| Keywords | CD Antigens & MHC (Veterinary), CD and Related Antigens (Human) |
| Notes | CD88 / C5aR is a G protein-coupled seven membrane-spanning protein serving as a receptor for C5a component of the complement cascade, and is expressed mainly by monocytes, macrophages, neutrophils, eosinophils, and mast cells, but also e.g. by hepatocytes, glial cells, vascular endothelial cells, or cardiomyocytes. The binding of C5a to CD88 is associated with inflammatory response, including superoxide anion production, chemotaxis, and increased production of acute phase proteins. Expression of CD88 on synovial mast cells and their C5a-mediated degranulation plays a role in pathogenesis of rheumatoid arthritis. |
| Alias Names | C5A, C5R1, C5AR1 |
| Previous Article No | AFC-5317-2 |
| Product Type | Antibodies Primary |
| Protocol | Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests. |
| Purification | Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography. |
| Research area | Immunology |
| Size | 100 tests |
| Source / Host | mouse |
| Species Reactivity | rabbit, mammals, cow/bovine, human |
| Storage | Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze. |
| Substrate / Buffer | Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
| Technical Specifications | The mouse monoclonal antibody S5/1 recognizes an extracellular epitope of CD88 protein, a 43 kDa receptor of C5a component of the complement cascade. |
| UniProt Number | P21730 |
| Product Page Updated | 2024-01-03T12:18:20.318Z |
