Article No
AFC-EA0UXA-100
Specifications
| Application | FC |
| Article No | AFC-EA0UXA-100 |
| Biosite Brand | Biosite Flow |
| Country Availability | all |
| Clone | NOK-1 |
| Clone Type | monoclonal |
| Conjugation | PE |
| Description | Anti-Hu CD178 PE |
| Recommended Dilution | The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary. |
| Supplier | Nordic Biosite |
| Entrez Gene ID | 356 |
| Format | PE |
| Immunogen | L5178Y mouse T lymphoma cells expressing recombinant human CD178 |
| Isotype | IgG1 |
| Keywords | Apoptosis (Human), CD and Related Antigens (Human) |
| Notes | CD178 / Fas-L (Fas ligand, CD95L), a member of TNF family transmembrane glycoproteins, is responsible for induction of apoptosis in cells containing its receptor CD95 / Fas. The CD178-mediated apoptosis pathway has been implicated in peripheral tolerance, tissue pathology, and maintenance of the immune privileged sites. Defects in this interaction may be related to some cases of systemic lupus erythematosus (SLE). CD178 was also described as a co-stimulatory receptor for T-cell activation in mice in vivo. |
| Alias Names | APTL, FASL, FAS-ligand, CD95L, TNFSF6, TNLG1A |
| Previous Article No | AFC-5325-2 |
| Product Type | Antibodies Primary |
| Protocol | Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests. |
| Purification | Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography. |
| Research area | Cell Death |
| Size | 100 tests |
| Source / Host | mouse |
| Species Reactivity | human |
| Storage | Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze. |
| Substrate / Buffer | Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
| Technical Specifications | The mouse monoclonal antibody NOK-1 recognizes an extracellular epitope of CD178 / Fas-L, an approximately 40 kDa transmembrane glycoprotein expressed on neutrophils, monocytes, and activated T and NK cells. |
| UniProt Number | P48023 |
| Product Page Updated | 2024-01-03T12:18:20.318Z |
