Article No
AFC-9U3SYN-100
Specifications
| Application | FC |
| Article No | AFC-9U3SYN-100 |
| Biosite Brand | Biosite Flow |
| Country Availability | all |
| Clone | P1H12 |
| Clone Type | monoclonal |
| Conjugation | PE |
| Description | Anti-Hu CD146 PE |
| Recommended Dilution | The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary. |
| Supplier | Nordic Biosite |
| Entrez Gene ID | 4162 |
| Format | PE |
| Immunogen | cultured human umbilical cells |
| Isotype | IgG1 |
| Keywords | CD Antigens & MHC (Veterinary), CD and Related Antigens & MHC (Rodent), CD and Related Antigens (Human) |
| Notes | CD146, also known as MCAM (melanoma cell adhesion molecule) or MUC18, is a heavily glycosylated transmembrane glycoprotein with more than 50% of the mass from carbohydrates. It is expressed on epithelial and endothelial cells, fibroblasts, multipotent mesenchymal stromal cells, activated T cells and activated keratinocytes, and on some cancer cells, especially melanoma. The presence of CD146 on circulating blood cells has been confined to the activated T cells rather than circulating endothelial cells. CD146 mediates heterophilic cell adhesion and regulates monocyte transendothelial migration. |
| Alias Names | MelCAM, MCAM, MUC18 |
| Previous Article No | AFC-5339-2 |
| Product Type | Antibodies Primary |
| Protocol | Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests. |
| Purification | Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography. |
| Research area | Immunology |
| Size | 100 tests |
| Source / Host | mouse |
| Species Reactivity | rabbit, dog/canine, mouse, human |
| Storage | Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze. |
| Substrate / Buffer | Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
| Technical Specifications | The mouse monoclonal antibody P1H12 recognizes an extracellular epitope of CD146, a 118 kDa transmembrane glycoprotein expressed on epithelial and endothelial cells, fibroblasts, multipotent mesenchymal stromal cells, melanoma cells, activated T cells and activated keratinocytes. |
| UniProt Number | P43121 |
| Product Page Updated | 2024-01-03T12:18:20.318Z |
