Article No
AFC-VOGR11-100
Specifications
| Application | FC |
| Article No | AFC-VOGR11-100 |
| Biosite Brand | Biosite Flow |
| Country Availability | all |
| Clone | UIC2 |
| Clone Type | monoclonal |
| Conjugation | PE |
| Description | Anti-Hu CD243 PE |
| Recommended Dilution | The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary. |
| Supplier | Nordic Biosite |
| Entrez Gene ID | 5243 |
| Format | PE |
| Immunogen | NIH 3T3 cells transfected with human CD243 (MDR-1) cDNA |
| Isotype | IgG2a k |
| Keywords | CD and Related Antigens (Human) |
| Notes | CD243, also known as multidrug resistant protein 1 (MDR-1) or P-glycoprotein (Pgp) is an ATP binding cassette (ABC)-containing efflux transporter for xenobiotic lipophilic compounds with broad substrate specificity. It is responsible for decreased drug accumulation in multidrug-resistant cells and often mediates the development of resistance to anticancer drugs. This protein also functions as a transporter in the blood-brain barrier. It is expressed in many tissues, including the brain, liver, pancreas, testes, kidney, and blood (B, T, NK cells, but not monocytes). |
| Alias Names | P-Glycoprotein, CLCS, MDR1, PGY1, ABC20, GP170, ABCB1, ABC transporter |
| Previous Article No | AFC-53D352 |
| Product Type | Antibodies Primary |
| Protocol | Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests. |
| Purification | Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography. |
| Research area | Immunology |
| Size | 100 tests |
| Source / Host | mouse |
| Species Reactivity | human |
| Storage | Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze. |
| Substrate / Buffer | Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
| Technical Specifications | The mouse monoclonal antibody UIC2 recognizes an extracellular epitope on CD243 (MDR-1), an approximately 170 kDa ABC transporter expressed on hematopoietic stem cells, B, T, and NK cells, or on many multidrug resistant cancer cells. This antibody preferentially recognizes CD243 in the process of transporting substrate. |
| UniProt Number | P08183 |
| Product Page Updated | 2024-01-03T12:18:20.318Z |
