Article No
AFC-L4RH79-0.1
Specifications
| Application | FC, IP |
| Article No | AFC-L4RH79-0.1 |
| Biosite Brand | Biosite Flow |
| Country Availability | all |
| Clone | MEM-158 |
| Clone Type | monoclonal |
| Concentration | 1 mg/ml |
| Conjugation | Biotin |
| Description | Anti-Hu CD15 Biotin |
| Recommended Dilution | The purified antibody is conjugated with Biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin. |
| Supplier | Nordic Biosite |
| Entrez Gene ID | 2526 |
| Format | Biotin |
| Immunogen | Human granulocytes |
| Isotype | IgM |
| Keywords | Blood Group Antigens (Human), CD and Related Antigens (Human) |
| Notes | CD15 (Lewis x), also known as stage specific embryonic antigen-1 (SSEA-1) is a trisacharide determinant (3-fucosyl-N-acetyllactosamine) expressed on several glycolipids, glycoproteins and proteoglycans of various cell types, e.g. granulocytes, mast cells, monocytes, macrophages, cells of gastric mucosa, nervous system or various tumour cells. There are several structural relatives of Lewis x, e.g. sialyl-Lewis x or sulphated Lewis x. Cells with high surface expression of Le(x) antigen exhibit strong self-aggregation, based on calcium-dependent Le(x)-Le(x) interaction. This process is involved for example in embryo compaction or in autoaggregation of teratocarcinoma cells. Sialyl-Le(x) and its isomer sialyl-Le(a) are ligands of selectins. CD15 expression has been extensively used to confirm diagnosis of Hodgkin´s disease. |
| Alias Names | Lewis x Blood Group antigen, Le(x), SSEA-1, 3-fucosyl-N-acetyllactosamine |
| Previous Article No | AFC-4844-2, AFC-L4RH79-0.1 |
| Product Type | Antibodies Primary |
| Protocol | Flow cytometry: recommended dilution: 1-4 μg/ml. |
| Purification | Purified antibody is conjugated with biotin LC-NHS ester under optimum conditions and unconjugated antibody and free biotin are removed by size-exclusion chromatography. |
| Research area | Immunology |
| Size | 0.1 mg |
| Source / Host | mouse |
| Species Reactivity | human |
| Storage | Store at 2-8°C. Do not freeze. |
| Substrate / Buffer | Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide |
| Technical Specifications | The antibody MEM-158 reacts with CD15, a cell membrane molecule 3-fucosyl-N-acetyllactosamine (3-FAL) strongly expressed on the surface of granulocytes, monocytes, macrophages, mast cells; it is also present on Langerhans cells and some myeloid precursors cells. |
| UniProt Number | P22083 |
| Product Page Updated | 2024-01-03T12:18:20.318Z |
