Article No
AFC-LVBO6D-100
Application | FC |
Article No | AFC-LVBO6D-100 |
Biosite Brand | Biosite Flow |
Country Availability | all |
Clone | NP4D6 |
Clone Type | monoclonal |
Conjugation | PE/Cy7 |
Description | Anti-Hu CD203c PE-Cy™7 |
Supplier | Nordic Biosite |
Entrez Gene ID | 5169 |
Format | PE-Cy™7 |
Immunogen | HEK-293 cells transfected with human CD203c |
Isotype | IgG1 |
Notes | CD203c, also known as ENPP-3, is integral membrane ectoenzyme (ectonucleotide pyrophosphatase/phosphodiesterase 3), that hydrolyses nucleotide triphosphates and thus modulates purinergic signaling. CD203c is expressed mainly on activated basophils and mast cells. CD203c is upregulated in response to IgE-receptor cross-linking and is overexpressed on neoplastic mast cells in patients with systemic mastocytosis. Measurement of its induced enhancement on the plasma membrane is useful for diagnostics of allergies. |
Alias Names | ENPP3, B10, PDNP3, PD-IBETA |
Product Type | Antibodies Primary |
Protocol | Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests. Extracellular and intracellular staining. |
Purification | Purified antibody is conjugated with activated tandem dye of R-phycoerythrin-cyanine 7 (PE-Cy™7) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography. |
Size | 100 tests |
Source / Host | mouse |
Species Reactivity | human |
Storage | Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze. |
Substrate / Buffer | Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
Technical Specifications | The mouse monoclonal antibody NP4D6 reacts with an extracellular epitope of CD203c, a transmembrane ectoenzyme expressed on basophils and mast cells, and overexpressed upon their activation. |
UniProt Number | O14638 |
Product Page Updated | 2024-01-03T12:18:20.318Z |