Article No
AFC-YWPDAJ-100
Anti-Hu CD31 PE-Cy™5
Anti-Hu CD31 PE-Cy™5
Antibodies Primary
Specifications
| Application | FC |
| Article No | AFC-YWPDAJ-100 |
| Biosite Brand | Biosite Flow |
| Country Availability | all |
| Clone | MEM-05 |
| Clone Type | monoclonal |
| Conjugation | PE/Cy5 |
| Description | Anti-Hu CD31 PE-Cy™5 |
| Supplier | Nordic Biosite |
| Entrez Gene ID | 5175 |
| Format | PE-Cy™5 |
| Immunogen | Leukocytes of a patient suffering from LGL-type leukaemia |
| Isotype | IgG1 |
| Notes | CD31 (platelet endothelial cell adhesion molecule-1, PECAM-1) is an inhibitory coreceptor involved in regulation of T cell and B cell signaling by a dual immunoreceptor tyrosine-based inhibitory motif (ITIM) that upon associated kinases-mediated phosphorylation provide docking sites for protein-tyrosine phosphatases. CD31 is expressed ubiquitously within the vascular compartment and is located mainly at junctions between adjacent cells. N-terminal Ig-like domain of CD31 is responsible for its homophilic binding, which plays an important role in cell-cell interactions. CD31 is a multifunctional molecule with diverse roles in modulation of integrin-mediated cell adhesion, transendothelial migration, angiogenesis, apoptosis, negative regulation of immunoreceptor signaling, autoimmunity, macrophage phagocytosis, IgE-mediated anaphylaxis and thrombosis. It is one of key regulatory molecules in vascular system. |
| Alias Names | PECAM-1, EndoCAM, GPIIA, PECA1 |
| Product Type | Antibodies Primary |
| Protocol | Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests. |
| Purification | Purified antibody is conjugated with activated tandem dye of R-phycoerythrin-cyanine 5 (PE-Cy™5) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography. |
| Size | 100 tests |
| Source / Host | mouse |
| Species Reactivity | human |
| Storage | Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze. |
| Substrate / Buffer | Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
| Technical Specifications | The antibody MEM-05 reacts with CD31 (PECAM-1), a 130-140 kDa type I transmembrane glycoprotein expressed on monocytes, platelets, granulocytes, endothelial cells and stem cells of the myeloid lineage. |
| UniProt Number | P16284 |
| Product Page Updated | 2024-01-03T12:18:20.318Z |
