Article No
AG-25T-0117-C010
anti-Profilin, pAb (IG-706)
anti-Profilin, pAb (IG-706)
Antibodies Primary
Specifications
| Additional Information | Contains Positive Control: Human platelet protein (Prod. No. AG-35T-0001), 500µg supplied at 5mg/ml in SDS sample buffer. Use 5µl (25µg) per lane for Western blotting of tricine gels (13% acrylamide) or 15% Laemmli gels. |
| Application | ICC, IHC, WB |
| Article No | AG-25T-0117-C010 |
| Country Availability | SE, FI, DK, NO, IS, EE, LV, LT |
| Clone | polyclonal |
| Clone Type | polyclonal |
| Concentration | 100 ug/ml |
| Conjugation | Unconjugated |
| Description | anti-Profilin, pAb (IG-706) |
| Supplier | Adipogen Life Sciences |
| Entrez Gene ID | 5216 |
| Format | Liquid |
| Gene Symbol | PFN1 |
| Immunogen | Human full-length profilin. |
| Notes | Polyclonal Antibody. Recognizes human, mouse, rat, pig, bovine and marsupial profilin. Source: Rabbit. Applications: ICC, IHC, WB. Liquid. In PBS containing 1mg/ml BSA and 0.02% sodium azide. Profilin (PFN1) is a ubiquitous small (12-15kDa) phosphoinositide and poly-L-proline binding protein that plays a role in signal transduction pathways and actin filament dynamics. There are two mammalian profilins with similar biochemical properties. Whereas profilin I appears to be highly expressed in most tissues except for skeletal muscle, profilin II is predominantly expressed in brain and at lower levels also in skeletal muscle, uterus and kidney. Profilin is a mainly cytosolic protein with higher concentrations in dynamic membrane areas like the leading edge and ruffling membranes. Profilin binding to PIP2 interferes with PIP2 hydrolysis by soluble phospholipase C-gamma, an inhibition that can be overcome by tyrosine phosphorylation of PLC-gamma. Besides actin monomer sequestration and stimulation of actin nucleotide exchange, profilin can also promote cellular actin filament growth. Profilin is involved in the actin dependent intracellular motility of cytopathogenic bacteria, the regulation of cell adhesion and possibly also in linking the actin cytoskeleton and endocytosis. Profilin has been found to associate with defined complexes containing proteins such as Arp2/3 or the Rho/Rac pathways constituents ROCK-II and HEM2/NAP1. Defects in PFN1 are the cause of amyotrophic lateral sclerosis 18 (ALS18).|Profilin (PFN1) is a ubiquitous small (12-15kDa) phosphoinositide and poly-L-proline binding protein that plays a role in signal transduction pathways and actin filament dynamics. There are two mammalian profilins with similar biochemical properties. Whereas profilin I appears to be highly expressed in most tissues except for skeletal muscle, profilin II is predominantly expressed in brain and at lower levels also in skeletal muscle, uterus and kidney. Profilin is a mainly cytosolic protein with higher concentrations in dynamic membrane areas like the leading edge and ruffling membranes. Profilin binding to PIP2 interferes with PIP2 hydrolysis by soluble phospholipase C-gamma, an inhibition that can be overcome by tyrosine phosphorylation of PLC-gamma. Besides actin monomer sequestration and stimulation of actin nucleotide exchange, profilin can also promote cellular actin filament growth. Profilin is involved in the actin dependent intracellular motility of cytopathogenic bacteria, the regulation of cell adhesion and possibly also in linking the actin cytoskeleton and endocytosis. Profilin has been found to associate with defined complexes containing proteins such as Arp2/3 or the Rho/Rac pathways constituents ROCK-II and HEM2/NAP1. Defects in PFN1 are the cause of amyotrophic lateral sclerosis 18 (ALS18). |
| Alias Names | PFN1 |
| Product Type | Antibodies Primary |
| Protocol | Contains Positive Control: Human platelet protein (Prod. No. AG-35T-0001), 500µg supplied at 5mg/ml in SDS sample buffer. Use 5µl (25µg) per lane for Western blotting of tricine gels (13% acrylamide) or 15% Laemmli gels. |
| Purity | Antigen affinity purified. |
| Research area | Inflammation |
| Shipping Information | BLUE ICE |
| Size | 10 ug |
| Source / Host | rabbit |
| Species Reactivity | cow/bovine, human, mouse, pig/porcine, rat |
| Stability | Stable for at least 1 year after receipt when stored at -20°C. |
| Storage | -20°C, 4°C |
| Substrate / Buffer | Liquid. In PBS containing 1mg/ml BSA and 0.02% sodium azide. |
| Technical Specifications | Polyclonal Antibody. Recognizes human, mouse, rat, pig, bovine and marsupial profilin. Source: Rabbit. Applications: ICC, IHC, WB. Liquid. In PBS containing 1mg/ml BSA and 0.02% sodium azide. Profilin (PFN1) is a ubiquitous small (12-15kDa) phosphoinositide and poly-L-proline binding protein that plays a role in signal transduction pathways and actin filament dynamics. There are two mammalian profilins with similar biochemical properties. Whereas profilin I appears to be highly expressed in most tissues except for skeletal muscle, profilin II is predominantly expressed in brain and at lower levels also in skeletal muscle, uterus and kidney. Profilin is a mainly cytosolic protein with higher concentrations in dynamic membrane areas like the leading edge and ruffling membranes. Profilin binding to PIP2 interferes with PIP2 hydrolysis by soluble phospholipase C-gamma, an inhibition that can be overcome by tyrosine phosphorylation of PLC-gamma. Besides actin monomer sequestration and stimulation of actin nucleotide exchange, profilin can also promote cellular actin filament growth. Profilin is involved in the actin dependent intracellular motility of cytopathogenic bacteria, the regulation of cell adhesion and possibly also in linking the actin cytoskeleton and endocytosis. Profilin has been found to associate with defined complexes containing proteins such as Arp2/3 or the Rho/Rac pathways constituents ROCK-II and HEM2/NAP1. Defects in PFN1 are the cause of amyotrophic lateral sclerosis 18 (ALS18). |
| UniProt Number | P07737 |
| Product Page Updated | 2024-02-01T08:25:01.492Z |
