Article No
AG-40T-0015-C010
PARP-1 [ARTD1] (E988K Mutant) (human) (rec.) Control
PARP-1 [ARTD1] (E988K Mutant) (human) (rec.) Control
Enzymes
Specifications
| Article No | AG-40T-0015-C010 |
| Country Availability | SE, FI, DK, NO, IS, EE, LV, LT |
| Concentration | Lot dependent (0.2-0.5mg/ml) |
| Description | PARP-1 [ARTD1] (E988K Mutant) (human) (rec.) Control |
| Supplier | Adipogen Life Sciences |
| Entrez Gene ID | 142 |
| Format | Liquid |
| Gene Symbol | PARP1 |
| Notes | PARP-1 (ARTD1) is involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. PARP-1 positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150. It forms a complex with EEF1A1 and TXK that acts as a T-helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production. PARP-1 (E988K mutant) is an inactive form of PARP-1 which can be used as a control compound.|Protein. Human full-length inactive mutant E998K of PARP-1 is fused to a HA-tag and a His-tag. Source: Sf21 cells. Liquid. In 50mM TRIS-HCl, pH 7.5, containing 100mM sodium chloride and 50mM imidazole, 0.2% NP-40 and 10% glycerol. Purity: >95% (SDS-PAGE). PARP-1 (ARTD1) is involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. PARP-1 positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150. It forms a complex with EEF1A1 and TXK that acts as a T-helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production. PARP-1 (E998K mutant) is an inactive form of PARP-1 which can be used as a control compound. |
| Alias Names | Poly(ADP-ribose) Polymerase 1; ADP-ribosyltransferase Diphtheria Toxin-like 1; NAD(+) ADP-ribosyltransferase 1 |
| Product Type | Enzymes |
| Purity | >95% |
| Research area | Metabolism |
| Sequence | Human full-length inactive mutant E988K of PARP-1 is fused to a HA-tag and a His-tag. |
| Shipping Information | DRY ICE |
| Size | 10 ug |
| Source / Host | human |
| Species Reactivity | human |
| Stability | Stable for at least 6 months after receipt when stored at -80°C. |
| Storage | -20°C, -80°C |
| Substrate / Buffer | Liquid. In 50mM TRIS-HCl, pH 7.5, containing 100mM sodium chloride and 50mM imidazole, 0.2% NP-40 and 10% glycerol. |
| Technical Specifications | Protein. Human full-length inactive mutant E998K of PARP-1 is fused to a HA-tag and a His-tag. Source: Sf21 cells. Liquid. In 50mM TRIS-HCl, pH 7.5, containing 100mM sodium chloride and 50mM imidazole, 0.2% NP-40 and 10% glycerol. Purity: >95% (SDS-PAGE). PARP-1 (ARTD1) is involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. PARP-1 positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150. It forms a complex with EEF1A1 and TXK that acts as a T-helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production. PARP-1 (E998K mutant) is an inactive form of PARP-1 which can be used as a control compound. |
| UniProt Number | P09874 |
| Product Page Updated | 2024-02-01T08:25:01.492Z |
