Article No
AM-V9014M
pFucci-S/G2/M Green (Cloning vector)
pFucci-S/G2/M Green (Cloning vector)
Vectors & Plasmids
Specifications
| Accession Number | Accession Numbers: AB370333 |
| Application | Cloning |
| Article No | AM-V9014M |
| Country Availability | SE, FI, DK, NO, IS, EE, LV, LT, FO, GL |
| Description | pFucci-S/G2/M Green (Cloning vector) |
| Supplier | MBL |
| Notes | AM-V9014 pFucci-S/G2/M Green is a Cloning vector encoding CoralHue® humanized monomeric Azami-Green1 (hmAG1) fused to a part of human Geminin (hGeminin). “Fucci” stands for Fluorescent Ubiquitination-based Cell Cycle Indicator. Geminin is an inhibitor of the DNA replication licensing factor. It accumulates during the S, G2, and M phases, but is degraded during G1 phase by ubiquitin-mediated proteolysis. A part of hGeminin (1-110) is also degradable in a cell cycle dependent manner. CoralHue® hmAG1 sequence is codon-optimized for higher expression in mammalian cells. CoralHue® monomeric AG1 (mAG1) has been generated from tetrameric CoralHue® Azami-Green (AG). |
| Product Type | Vectors & Plasmids |
| Purity | A260/A280 > 1.5 |
| Shipping Information | 4°C |
| Size | 20 µg |
| Storage | -20°C |
| Substrate / Buffer | Dry form. Reconstitute with distilled water or TE before use. |
| Technical Specifications | AM-V9014 pFucci-S/G2/M Green is a Cloning vector encoding CoralHue® humanized monomeric Azami-Green1 (hmAG1) fused to a part of human Geminin (hGeminin). “Fucci” stands for Fluorescent Ubiquitination-based Cell Cycle Indicator. Geminin is an inhibitor of the DNA replication licensing factor. It accumulates during the S, G2, and M phases, but is degraded during G1 phase by ubiquitin-mediated proteolysis. A part of hGeminin (1-110) is also degradable in a cell cycle dependent manner. CoralHue® hmAG1 sequence is codon-optimized for higher expression in mammalian cells. CoralHue® monomeric AG1 (mAG1) has been generated from tetrameric CoralHue® Azami-Green (AG). |
| Product Page Updated | 2023-12-29T16:20:16.638Z |
