Immunohistochemical staining procedures often start with incubation of a blocking solution to reduce unspecific binding of primary antibody to tissue sections. This step can be omitted if the antibody used is diluted in Antibody Diluent. Antibody Diluent • minimises unspecific binding of the primary antibody to the tissue section, • reduces surface tension of the antibody solution and improves spreading the reagent on the slide, • increases microbial and chemical stability of the antibody, • reduces adhesion of antibody to the surface of the vial, • and minimises the danger of antibody degradation by proteolytic enzymes.
Elias JM “Immunohistopathology – A practical Approach to Diagnosis” ASCP Press 2003 Nadji M and Morales AR Ann N.Y. Acad Sci 420:134-9, 1983
The solution should be stored at 2-8°C without further dilution. Do not freeze it.
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