Article No
BIO-52072
ISOLATE II RNA Mini Kit
ISOLATE II RNA Mini Kit
Kits & Panels
Specifications
| Application | RNA Purification |
| Article No | BIO-52072 |
| Country Availability | SE, DK, NO, IS, EE, LV, LT |
| Description | ISOLATE II RNA Mini Kit |
| Supplier | Bioline Reagents |
| Keywords | Isolate RNA / DNA Purification Kits |
| Notes | ISOLATE II RNA Mini Kit is specially designed for the fast and efficient isolation of extremely pure total RNA from a variety of sources. The kit is compatible with animal tissues, cultured cells, bacterial cells, yeast and biological fluids (cell-free). Biological samples which are sometimes difficult to process will yield high-quality RNA i.e. mouse tissue (liver, brain), various tumour cell lines, Streptococcus and Actinobacillus pleuropneumoniae. The sample is lysed with an optimized lysis buffer containing chaotropic ions, which simultaneously inactivates RNases, preventing degradation of the released RNA. The lysate is then applied to a spin column to selectively remove genomic DNA (RNase-free DNase I is supplied with the kit). There is no need to perform a separate DNase digestion step. The RNA is bound to the silica membrane, washed and pure, high quality RNA is eluted (Fig. 1). The online product manual has protocols for purifying total RNA from cultured cells, tissues, yeast, bacteria, biological liquids, paraffin embedded tissue and RNAlater® treated samples, as well as DNase I treatment of purified RNA and clean-up of RNA from reaction mixtures. The isolated RNA shows excellent performance in downstream applications such as reverse transcription, one-step real-time PCR (Fig. 2), Northern blot analysis, microarrays and RNA protection assays. Additional Lysis Buffer RLY can be ordered separately (BIO-52079) for processing higher amounts of starting materials. |
| Product Type | Kits & Panels |
| Research area | Molecular Biology |
| Size | 50 Preps |
| Product Page Updated | 2023-12-29T09:10:16.397Z |