Kits & Panels

JetSeq Library Quantification Hi-ROX Kit

NEW
JetSeq Library Quantification Hi-ROX Kit

Article No

BIO-68028

Size

500 Reactions

Specifications

Article No BIO-68028
Country Availability SE, DK, NO, IS, RU
Description JetSeq Library Quantification Hi-ROX Kit
Supplier Bioline Reagents
Notes Accurate quantification of the number of amplifiable library molecules loading into a flow cell is one of the most critical steps in the next-generation sequencing (NGS) workflow in obtaining high-quality read data with NGS technologies. Loading insufficient amount of library DNA will result in low cluster density and reduced sequencing yield. An overabundance of library DNA may increase cluster density and result in poor quality data. Standard methods of NGS library quantification, by electrophoresis or spectrophotometry, have low sensitivity, are non-specific for adapter-bound DNA and typically require a large amount of library sample for analysis. With its greater sensitivity and broad dynamic range, qPCR is therefore seen as the gold standard for NGS library quantification, as it accurately measures the number of molecules that can serve as templates during library and cluster amplification, even with very dilute libraries. Bioline has developed the JetSeq™ Library Quantification Kit, an optimized, robust SYBR® Green based qPCR kit that provides accurate quantification of Illumina based NGS libraries. The JetSeq Library Quantification Kit contains pre-diluted standards to minimize pipetting errors, a pre-qualified P5 and P7 Illumina adaptor sequence primer mix to ensure reproducible and precise qPCR results and an optimized buffer for dilution of NGS library samples.
Product Type Kits & Panels, Library Preparation
Research Area Molecular Biology
Size 500 Reactions
Product Page Updated 2021-04-15T08:03:21.644Z

Shipping info

The delivery time for this item is approximately 5-8 business days. 
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