Article No
BPS-71101
Carbonic Anhydrase 9 (CA9), His-tag Recombinant
Carbonic Anhydrase 9 (CA9), His-tag Recombinant
Proteins & Peptides
Specifications
| MW | 39.7 kDa |
| Accession Number | NM_001216 |
| Additional Information | CA IX is a transmembrane protein and the only tumor-associated carbonic anhydrase isoenzyme known. It is expressed in all clear-cell renal cell carcinoma, but is not detected in normal kidney or most other normal tissues. Expression of the membrane-bound carbonic anhydrase, CA IX, has been reported in hypoxic tumors where its activity is linked to promoting survival of tumor cells and aiding cancer progression. This is thought to occur through the carbonic anhyrase-mediated production of bicarbonate ions, which neutralizes the abnormally acidic pH of the extracellular environment induced during hypoxia. Furthermore, as CA IX is a hypoxia- and HIF-1-inducible enzyme, its expression can be used as a biomarker for hypoxic tumors. |
| Article No | BPS-71101 |
| Country Availability | SE, FI, DK, NO, IS, EE, LV, LT |
| Description | Carbonic Anhydrase 9 (CA9), His-tag Recombinant |
| Supplier | BPS Bioscience |
| Entrez Gene ID | 768 |
| Format | Aqueous buffer solution |
| Gene Symbol | CA9 |
| Notes | Human carbonic anhydrase IX (CA IX), also known as CA9, (GenBank Accession No. NM_001216), a.a. 59-414 with C-terminal His-tag, MW = 39.7 kDa, expressed in a baculovirus infected Sf9 cell expression system. |
| Alias Names | Carbonic Anhydrase IX, PMW, RCC-Associated Antigen G250, CAIX, Carbonic Anhydrase 9, Carbonate Dehydratase, CA9 |
| Product Type | Proteins & Peptides |
| Protocol | 15 mM Tris-HCl, pH 7.4, 100 mM NaCl, and 3 mM p-Nitrophenyl acetate (PNPA). Incubate for 30 minutes at room temperature. Absorbance is measured at 405 nM. |
| Find out more about our research areas Biochemical, Immunology, Molecular Biology and Pathology | Metabolism |
| Shipping Information | -80°C (dry ice) |
| Size | 100 µg |
| Species Reactivity | human |
| Storage | At least 6 months at -80°C. |
| Substrate / Buffer | 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 266 mM imidazole and 20% glycerol |
| Technical Specifications | Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. |
| UniProt Number | Q16790 |
| Product Page Updated | 2024-01-09T13:23:45.900Z |
