Article No
BPS-79037
14-3-3 sigma Protein, His-tag (Human) Recombinant
14-3-3 sigma Protein, His-tag (Human) Recombinant
Proteins & Peptides
Specifications
| MW | 30 kDa |
| Accession Number | NM_006142 |
| Additional Information | 14-3-3 sigma or stratifin is a protein that is strongly induced by gamma irradiation and other DNA-damaging agents (1). The induction of 14-3-3 sigma is mediated by a p53 responsive element. Exogenous introduction of 14-3-3 sigma into cycling cells results in a G2 cell cycle arrest (2). Knockout of 14-3-3 sigma in cells showed that the cells are unable to maintain cell cycle arrest after DNA damage. The 14-3-3sigma -/- cells die as they enter mitosis. This process is associated with a failure of the 14-3-3sigma-deficient cells to sequester the proteins that initiate mitosis and prevent them from entering the nucleus. Thus, 14-3-3 sigma plays an important role in maintaining the G2 checkpoint in cells and preventing mitotic death. |
| Application | WB |
| Article No | BPS-79037 |
| Country Availability | SE, FI, DK, NO, IS, EE, LV, LT |
| Description | 14-3-3 sigma Protein, His-tag (Human) Recombinant |
| Supplier | BPS Bioscience |
| Entrez Gene ID | 2810 |
| Format | Aqueous buffer solution |
| Gene Symbol | SFN |
| Notes | Human 14-3-3 sigma Protein, GenBank Accession No. NM_006142, full length, with N-terminal His-tag, expressed in Sf9 insect cells using baculovirus. MW = 30 kDa. |
| Alias Names | 14-3-3 sigma, SFN, stratifin, YWHAS |
| Product Type | Proteins & Peptides |
| Shipping Information | -80°C (dry ice) |
| Size | 20 µg |
| Species Reactivity | human |
| Storage | Store product at -80°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles. |
| Substrate / Buffer | 50 mM sodium phosphate, pH 7.0, 300 mM NaCl, 150 mM imidazole, 0.1 mM PMSF, 0.25 mM DTT, 25% glycerol |
| Technical Specifications | Western blot |
| UniProt Number | P31947 |
| Product Page Updated | 2024-01-09T13:23:45.900Z |
