Enzymes

A20, FLAG-tag (Sf9-derived)

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A20, FLAG-tag (Sf9-derived)

Article No

BPS-80394

Size

50 ug

Shipping Information

DRY ICE

Documentation

Specifications

MW 90.5 kDa
Accession Number NM_006290
Additional Information Avoid freeze/thaw cycles
Article No BPS-80394
Country Availability SE, FI, DK, NO, IS, EE, LV, LT, RU
Description A20, FLAG-tag (Sf9-derived)
Supplier BPS Bioscience
Entrez Gene ID 7128
Gene Symbol TNFAIP3
Notes Human A20, also known as tumor necrosis factor-induced protein 3 (TNFAIP3) or OTU domain-containing protein 7C (OTUD7C), GenBank Accession No. NM_006290, a.a. 2-790(end) with an N-terminal FLAG-tag, MW=90.5 kDa, expressed in Sf9 insect cells via a baculovirus expression system.
Alias Names A20, tumor necrosis factor-induced protein 3, TNFAIP3, OTU domain-containing protein 7C, OTUD7C
Product Type Enzymes
Protocol Reaction was performed in 50 mM Tris pH 7.4, 1 mM DTT, 0.5 mM EDTA, 500 nM Ub-AMC, and various amount of A20 in a volume of 50 µL. Reaction was incubated at 37°C for 30 min. and fluorescent signal was measured at excitation of 360 nm, and emission at 460 nm.
References 1. Shi CS, Kehrl JH. (2010). Autophagy 6(7):986-7.<br>2. Tavares, R.M., et al. (2010). Immunity 33(2):181-9.<br>3. Hymowitz, S.G., Wertz, I.E. (2010). Nat. Rev. Cancer 10(5):332-41
Shipping Information DRY ICE
Size 50 ug
Storage -80°C
Substrate / Buffer 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 0.05% _x000D_ Tween-20, 20% glycerol, and 80 µg/ml FLAG peptide
Technical Specifications Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
UniProt Number P21580
Product Page Updated 2021-01-05T12:59:29.990Z

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