A20, His tag, FLAG-tag (Sf9-derived)

A20, His tag, FLAG-tag (Sf9-derived)

Article No



50 ug

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MW 91 kDa
Accession Number NM_006290
Additional Information Avoid freeze/thaw cycles
Article No BPS-80408
Country Availability SE, FI, DK, NO, IS, EE, LV, LT, RU
Description A20, His tag, FLAG-tag (Sf9-derived)
Supplier BPS Bioscience
Entrez Gene ID 27030
Gene Symbol MLH3
Notes Human A20, also known as tumor necrosis factor- induced protein 3 (TNFAIP3) or OTU domain-containing protein 7C (OTUD7C), GenBank Accession No. NM_006290, amino-acids 2-790(end) with an N-terminal FLAG-tag and C-terminal His-tag, MW=91 kDa, expressed in Sf9 insect cells via a baculovirus expression system.
Alias Names tumor necrosis factor-induced protein 3, TNFAIP3, OTU domain-containing protein 7C, OTUD7C, A20
Product Type Enzymes
Protocol Reaction was performed_x000D_ in 50 mM Tris pH 7.4, 1 mM DTT, 0.5 mM_x000D_ EDTA, 500 nM Ub-AMC, and various_x000D_ amount of A20 in a volume of 50 µL._x000D_ Reaction was incubated at 37°C for 30 min_x000D_ and fluorescent signal was measured at_x000D_ excitation of 360 nm, and emission at 460_x000D_ nm.
References 1. Shi CS, Kehrl JH. (2010). Autophagy 6(7):986-7.<br>2. Tavares, R.M., et al. (2010). Immunity 33(2):181-9.<br>3. Hymowitz, S.G., Wertz, I.E. (2010). Nat. Rev. Cancer 10(5):332-41
Shipping Information DRY ICE
Size 50 ug
Storage -80°C
Substrate / Buffer 40 mM Tris-HCl, pH 8.0,_x000D_ 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-_x000D_ 20, 3 mM DTT, and 20% glycerol
Technical Specifications Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
UniProt Number Q9UHC1
Product Page Updated 2021-01-05T12:59:29.990Z

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