Library Preparation

Zymo-Seq RiboFree Total RNA Library Kit (96 preps)

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Zymo-Seq RiboFree Total RNA Library Kit (96 rxn)

Article No

BioSite-R3003

Size

96 preparations

Shipping Information

Room Temp & Dry Ice Items

Documentation

Specifications

Article No BioSite-R3003
Country Availability SE, FI, DK, NO, FO, GL
Description Zymo-Seq RiboFree Total RNA Library Kit (96 rxn)
Supplier Zymo Research
Notes Zymo-Seq RiboFree Total RNA-Seq Library Kit is the fastest, easiest RNA-Seq library prep kit available to make stranded, total RNA libraries. The automation-friendly protocol minimizes hands-on time to generate sequencer-ready, stranded, indexed libraries depleted of rRNA and globin in as little as 3.5 hours. Depletion of ribosomal RNAs (which can comprise as much as 90% of a total RNA sample) is completely integrated into the workflow, using a novel, probe-free technology that removes rRNA, globin, or any other over-abundant, repetitive transcript from any sample type or species. RiboFree Universal Depletion is validated across biological kingdom and phyla, including human, rodent, plant, and various prokaryotes, as well as RNA from a wide range of sample types including cells, tissue, FFPE tissues, and whole blood, eliminating the need to select sample-specific modules for different sample types. Just one total RNA-Seq library prep kit to deplete them all. Because the RiboFree Universal Depletion is probe-free, researchers using the Zymo-Seq RiboFree Total RNA Library Kit will avoid transcriptome profiling bias found in other methods. High concentrations of rRNA-binding oligonucleotides used in probe-based approaches also bind to a significant number of non-ribosomal targets. As much as 20% of the 20,000 protein-coding genes in the human genome are significantly biased by these methods. In contrast, the probe-free depletion developed by Zymo Research uses only abundant pre-existing transcripts for mismatch-free enzymatic removal of rRNA and globin, resulting in minimal off-target effects as low as 1.8% in all protein-coding transcripts. This total RNA-Seq library prep kit's protocol is fragmentation-free and works with up to 2 ug of undepleted total RNA, down to 50 ng of undepleted total RNA, or as little as 5 ng of ribosome-depleted RNA samples. Pre-mixed reagents are ready-to-use at every step, eliminating the need for manual master-mix preparation. Everything needed to make sequencing-ready libraries, including indexing primers, SPRI beads, and magnetic bead stand are included in the kit. It's RNA-Seq made simple.
Product Type Library Preparation
Shipping Information Room Temp & Dry Ice Items
Size 96 preparations
Storage -70C & RT
Product Page Updated 2021-04-16T13:33:11.433Z

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The delivery time for this item is approximately 4-7 business days, depending on the availability at manufacturer. If other deviations occur, this will be noted on the order confirmation or communicated via email. Please note, we do reserve the right to select the best packaging and shipping method in order to insure the stability of the product and allow efficient order tracking.