Article No
CDX-G0061-L250
Gram's Safranin Solution
Gram's Safranin Solution
Chemicals & Biochemicals
Specifications
| CAS No | 477-73-6 |
| MW | 350.84 |
| Article No | CDX-G0061-L250 |
| Country Availability | SE, FI, DK, NO, IS, EE, LV, LT |
| Description | Gram's Safranin Solution |
| Supplier | Adipogen Life Sciences |
| Format | Liquid |
| Notes | Chemical. CAS: 477-73-6. Formula: C20H19ClN4. MW: 350.84. Synthetic. Gram Safranin Solution is a reagent recommended for use in qualitative procedures to differentiate gram-negative from gram-positive organisms. The Gram staining allows a fast differentiation of bacteria in Gram-positive and Gram-negative. The mureine structure of the bacteria walls is the basis of the color affinity. Bacteria will be stained with Gram's crystal violet solution - an aniline dye - in the first step. After the treatment with iod solution, a dye-iod complex will form. During the decolorizing step, this complex stays in the multilayer mureine structures of the Gram-positive bacteria and they will appear blue. Gram-negative bacteria have a monolayer mureine structure only, the dye-iod complex does not stay bound to the cellwall, they will be decolorized. Gram-negative bacteria will be counterstained by safranin solution and appear orange.|Gram Safranin Solution is a reagent recommended for use in qualitative procedures to differentiate gram-negative from gram-positive organisms. The Gram staining allows a fast differentiation of bacteria in Gram-positive and Gram-negative. The mureine structure of the bacteria walls is the basis of the color affinity. Bacteria will be stained with Gram's crystal violet solution - an aniline dye - in the first step. After the treatment with iod solution, a dye-iod complex will form. During the decolorizing step, this complex stays in the multilayer mureine structures of the Gram-positive bacteria and they will appear blue. Gram-negative bacteria have a monolayer mureine structure only, the dye-iod complex does not stay bound to the cellwall, they will be decolorized. Gram-negative bacteria will be counterstained by safranin solution and appear orange. |
| Molecular Formula | C20H19ClN4 |
| Alias Names | Safranin O Solution; Safarin T Solution |
| Product Type | Chemicals & Biochemicals |
| Purity | for microscopy |
| Shipping Information | RT |
| Size | 250 ml |
| Solubility | Soluble in ethanol. |
| Stability | Stable for at least 2 years after receipt when stored at RT. |
| Storage | RT |
| Technical Specifications | Chemical. CAS: 477-73-6. Formula: C20H19ClN4. MW: 350.84. Synthetic. Gram Safranin Solution is a reagent recommended for use in qualitative procedures to differentiate gram-negative from gram-positive organisms. The Gram staining allows a fast differentiation of bacteria in Gram-positive and Gram-negative. The mureine structure of the bacteria walls is the basis of the color affinity. Bacteria will be stained with Gram's crystal violet solution - an aniline dye - in the first step. After the treatment with iod solution, a dye-iod complex will form. During the decolorizing step, this complex stays in the multilayer mureine structures of the Gram-positive bacteria and they will appear blue. Gram-negative bacteria have a monolayer mureine structure only, the dye-iod complex does not stay bound to the cellwall, they will be decolorized. Gram-negative bacteria will be counterstained by safranin solution and appear orange. |
| Product Page Updated | 2024-02-01T08:25:01.492Z |
