Article No
CSB-MA000071M1m-100
Specifications
| Application | ELISA, IF, IHC, IP, WB |
| Article No | CSB-MA000071M1m-100 |
| Country Availability | SE, FI, DK, NO, IS, EE, LV, LT, FO, GL |
| Clone | 10B4E3 |
| Clone Type | monoclonal |
| Conjugation | Unconjugated |
| Description | GAPDH Monoclonal Antibody |
| Supplier | Cusabio |
| Immunogen | Recombinant Human GAPDH protein (2-335AA) |
| Isotype | IgG1 |
| Notes | Glyceraldehyde 3-phosphate dehydrogenase (GAPDH or G3PDH) is an enzyme of 37kDa that is consisdered as a cellular enzyme involved in glycolysis. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a pleiotropic enzyme that is overexpressed in apoptosis and in several human chronic pathologies. Its role as a mediator for cell death has also been highlighted. At the molecular level, sequential steps lead to nuclear translocation of GAPDH during cell death as follows: first, a catalytic cysteine in GAPDH (C150 in rat GAPDH) is S-nitrosylated by nitric oxide (NO) that is generated from inducible nitric oxide synthase (iNOS) and/or neuronal NOS (nNOS); second, the modified GAPDH becomes capable of binding with Siah1, an E3 ubiquitin ligase, and stabilizes it; third, the GAPDH-Siah protein complex translocates to the nucleus, dependent on Siah1's nuclear localization signal, and degrades Siah1's substrates in the nucleus, which results in cytotoxicity. A recent report suggests that GAPDH may be genetically associated with late-onset of Alzheimer's disease.-deprenyl, which has originally been used as a monoamine oxidase inhibitor for Parkinson's disease, binds to GAPDH and displays neuroprotective actions. |
| Product Type | Antibodies Primary |
| Purification | Protein G Purified |
| Shipping Information | BLUE ICE |
| Size | 100ul |
| Source / Host | mouse |
| Species Reactivity | human, mouse, rabbit |
| Storage | -80°C |
| Product Page Updated | 2024-01-24T13:11:22.259Z |
