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Acidic Mammalian Chitinase (Human, Active)
Acidic Mammalian Chitinase (Human, Active)
Proteins & Peptides
On Request
Specifications
| MW | 56 kDa |
| Article No | CY-E1248 |
| Country Availability | SE, FI, DK, NO, IS, EE, LV, LT, FO, GL |
| Components | Human acidic mammalian chitinase containing a C-terminal His-tag, expressed in HEK293 cells. |
| Concentration | 0.2 μg/μL |
| Description | Acidic Mammalian Chitinase (Human, Active) |
| Supplier | MBL |
| Additional Information | Human acidic mammalian chitinase containing a C-terminal His-tag, expressed in HEK293 cells and purified by nickel chelating agarose chromatography. |
| Notes | The glycosyl hydrolase family is quite large, with over 100 families. Acidic mammalian chitinase (AMCase) and chitotriosidase-1 are enzymatically active true chitinases in a member of the glycosyl hydrolase-18 family, identified by EC 3.2.1.14. In mammals, only these two enzymes cleave N-acetyl-beta-D-glucosamine (1->4)-beta linkages in chitodextrins and chitin, the second most abundant biopolymer that can be found in the cell walls of fungi, micro-filarial sheaths of helminths, and exoskeletons of insects and crustaceans (1). Other member included in this family are chitinase-like molecules, e.g. YKL-40/Chitinase-3-like-1 and YKL-39/chitinase-3-like-2, that are thought to lack the ability to hydrolyze the chitin linkages, but retain chitin-binding activity (2, 3) and act more like connective tissue proteins, and immediate early gene products. AMCase was cloned as an acid-stable active enzyme and is highly expressed in the gastrointestinal tract, and to a lesser extent, in the lung and alveolar macrophages in both humans and mice (4, 5), with an optimum activity at pH 4–5 (6). AMCase as well as chitotriosidase-1 is 50 kDa proteins, consisting of a 39 kDa catalytic region separated from a chitin-binding domain by a hinge region (7,8). They both show considerable homology to chitinases from lower organisms. AMCase is one of the important proteins involved in Th2-mediated inflammation and has been implicated in asthma and allergic diseases by affecting the IL-13 downstream pathway (9). Inhibition of AMCase results in decreased airway inflammation and airway hyper-responsiveness in a murine asthmatic model, suggesting that the AMCase activity is a part of the mechanism of Th2 cytokine-driven inflammatory response in asthma (9). |
| Alias Names | CHIA, AMCase |
| Product Type | Proteins & Peptides |
| Purity | > 90 % as determined by SDS PAGE |
| Research area | Immunology |
| Shipping Information | Dry Ice |
| Size | 10 µg |
| Species Reactivity | mammals |
| Stability | 1 year |
| Storage | -70°C |
| Substrate / Buffer | Recombinant human acidic mammalian chitinase is supplied frozen in a buffer containing 20 mMHEPES-KOH, pH 7.5, 1 mM DTT, 50 mM NaCl and 50% glycerol. |
| Technical Specifications | The glycosyl hydrolase family is quite large, with over 100 families. Acidic mammalian chitinase (AMCase) and chitotriosidase-1 are enzymatically active true chitinases in a member of the glycosyl hydrolase-18 family, identified by EC 3.2.1.14. In mammals, only these two enzymes cleave N-acetyl-beta-D-glucosamine (1->4)-beta linkages in chitodextrins and chitin, the second most abundant biopolymer that can be found in the cell walls of fungi, micro-filarial sheaths of helminths, and exoskeletons of insects and crustaceans (1). Other member included in this family are chitinase-like molecules, e.g. YKL-40/Chitinase-3-like-1 and YKL-39/chitinase-3-like-2, that are thought to lack the ability to hydrolyze the chitin linkages, but retain chitin-binding activity (2, 3) and act more like connective tissue proteins, and immediate early gene products. AMCase was cloned as an acid-stable active enzyme and is highly expressed in the gastrointestinal tract, and to a lesser extent, in the lung and alveolar macrophages in both humans and mice (4, 5), with an optimum activity at pH 4–5 (6). AMCase as well as chitotriosidase-1 is 50 kDa proteins, consisting of a 39 kDa catalytic region separated from a chitin-binding domain by a hinge region (7,8). They both show considerable homology to chitinases from lower organisms. AMCase is one of the important proteins involved in Th2-mediated inflammation and has been implicated in asthma and allergic diseases by affecting the IL-13 downstream pathway (9). Inhibition of AMCase results in decreased airway inflammation and airway hyper-responsiveness in a murine asthmatic model, suggesting that the AMCase activity is a part of the mechanism of Th2 cytokine-driven inflammatory response in asthma (9). |
| Product Page Updated | 2024-01-10T15:49:33.450Z |
