AEC Chromogenic kit
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|Country Availability||SE, FI, DK, NO, IS, EE, LV, LT|
|Data Sheet Link||http://www.genetex.com/Web/Product/ProductToPDF.aspx?No=GTX16204&Country=46|
|Description||AEC Chromogenic kit|
|Notes||AEC is one of the most important chromogenic reagents for peroxidase. The coloration which is showed to be fresh red, and applies to immunohistochemistry and various dot detection. Easy to solution in organic impregnant is its defect, to seal slide with water soluble sealing reagent in case of using AEC is recommend.|
|Protocol||1. After the peroxidase detection, wash 4 times with TBS or PBS and each time for 5 minutes.<br>2. Add one drop of chromogenic reagent A and B respectively into 1ml of distilled water and mix evenly Add it to the sample and colorate in dark for 10-30 minutes. If no background appearing, keep coloration until satisfied.<br>3. Wash fully with distilled water to stop reaction. Counterstain with methyl blue for 3-5 minutes or nonalcoholic hematoxylin (Mayer's) if necessary.<br>4. Seal sllide with water soluble sealing reagent (Boster can provide), or glycerol. Observe with microscope. Counterstain with alcoholic hematoxylin is not recommended.|
|Storage||Store at -20°C for one year.|
|Technical Specifications||For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.|
|Product Page Updated||2019-01-17T12:56:28.093Z|
The delivery time for this item is approximately 7-10 business days. If other deviations occur, this will be noted on the order confirmation or communicated via email. Please note, we do reserve the right to select the best packaging and shipping method in order to insure the stability of the product and allow efficient order tracking.