Most fixatives immobilize the tissue antigens by cross linkages. Sometimes, extensive cross linking of proteins in high concentrations can also lead to the masking or destruction of antigens, which leads to poor staining or no staining at all. This loss of staining can be restored to a certain extent by pretreatment of tissue sections with proteolytic enzymes. The unmasking of tissue sections is an important requirement for the optimal staining of antigens like immunoglobulins, keratins, carcinoembryonic antigen, factor VIII related antigen, testosterone, and to a lesser extent S100 protein.
This antigen retrieval solution is provided in a ready-to-use format requiring no further preparation. 1.) Apply this solution to tissue sections following their initial hydration step. Incubate for 10 min at 37ºC or 45ºC (this incubation time may vary depending on the degree of fixation of the tissue sections. 2.) Wash tissue sections well with IHC wash buffer (GTX28195), or PBS or TBS if immunostaining by hand.
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
The delivery time for this item is approximately 7-10 business days. If other deviations occur, this will be noted on the order confirmation or communicated via email. Please note, we do reserve the right to select the best packaging and shipping method in order to insure the stability of the product and allow efficient order tracking.