Sample Preparation

Antigen retrieval condition-Pepsin (ready to use)

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Antigen retrieval condition-Pepsin (ready to use)

Article No

GTX30935

Application

IHC

Size

100 ml

Documentation

Suggested protocols

Specifications

Application IHC
Article No GTX30935
Country Availability SE, FI, DK, NO, IS, EE, LV, LT
Description Antigen retrieval condition-Pepsin (ready to use)
Supplier GeneTex
Format Liquid
Notes Pepsin is used for proteolytic digestion of formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies. In IHC most commonly used fixative like formalin mask tissue antigens (cellular, membrane and nuclear) by cross-linking process, this results in poor or no staining in IHC. Pepsin digestion of FFPE tissue section improves accessibility of antibodies to tissue antigens.
Product Type Sample Preparation
Protocol Deparafinize FFPE tissue section is carried out as outlined in your protocol. Hydrate tissue with PBS. Remove buffer, add 2-4 drops of Pepsin reagent to cover tissue section. Incubate at 37oC or at 56oC for 5-10 minutes (the time required for optimum digestion of FFPE tissue section will vary with the extent of fixation, generally approx. 5-10 minutes are sufficient at the above temperatures. In some cases it may require 15 minute.). Discard Pepsin reagent, wash 3-5 times with buffer.
Size 100 ml
Storage 4°C
Technical Specifications For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Product Page Updated 2021-03-03T13:11:18.050Z

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