Creatinine Urinary Detection Kit (2 Plate Kit)

Article No K002-H1
Product Type Assay & Detection
Size 2x96 well plates

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Country Availability DK, EE, FI, IS, LT, LV, NO, SE
Data Sheet Link
Description Creatinine Urinary Detection Kit (2 Plate Kit)
Supplier Arbor Assays Inc.
Notes The DetectX™ Urinary Creatinine kit measures creatinine present in diluted urine samples. A creatinine standard, calibrated to a NIST creatinine standard, is provided to generate a standard curve. Standards or samples are pipetted into the clear microtiter plate. The color generating reaction is initiated with the DetectX™ Creatinine Reagent added to all standards, controls and samples. After a short incubation the intensity of the generated color is read in a microtiter plate reader at 490nm. Creatinine is a metabolite of phosphocreatine (p-creatine), a molecule used as a store for high-energy phosphate for the production of ATP. Creatine and p creatine are converted non-enzymatically to the metabolite creatinine, which diffuses into the blood and is excreted by the kidneys. Under normal conditions, its formation occurs at a rate that is relatively constant and so creatinine is a useful tool for normalizing the levels of other molecules found in urine.
Protocol Urine samples should be diluted 1:20 with deionized or distilled water. • Allow the kit reagents to come to room temperature for 30 minutes. • Pipet 50 μL of diluted samples, water as the blank, or standards into wells in the clear plate. • Add 100 μL of the DetectX™ Creatinine Reagent to each well. • Incubate at room temperature for 30 minutes. • Read the optical density in a plate reader at 490nm.
References 1. Wallimann, T. et al., Biochem. J., 2000, 281, 21-40. 2. Wyss, M. and Kaddurah-Daouk, R., Physiol. Rev., 2000, 80, 1107-1213. 3. Raja Iyengar, M. et al., J. Biol. Chem, 1985, 260, 7562-7567. 4. Manjunath, G. et al., Postgrad. Med. 2001, 110, 55-62. 5. Gross, J.L. et al., Diabetes Care, 2005, 28, 164-176. 6. Anavekar, N.S. et al., New Engl. J. Med., 2004, 351, 1285-1295
Species Reactivity human, monkey, mouse, rat, dog/canine
Technical Specifications A diluted urine sample was spiked with 2,000 mg/dL of glucose. The unspiked diluted sample read at 8.44 mg/dL. No significant change to the measured creatinine level was seen at any glucose concentration. This assay has been validated for diluted human urine samples. The end user should evaluate recoveries of creatinine in other species being studied.
Product Page Updated 2019-01-03T09:12:03.477Z

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Species reactivity:

human, monkey, mouse, rat, dog/canine human monkey mouse rat

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Product data sheet