Article No
K006-F1D
Application | ELISA |
Article No | K006-F1D |
Country Availability | SE, FI, DK, NO, IS, EE, LV, LT |
Components | Detection |
Description | Glutathione Fluorescent 384-Well Detection Kit |
Detection Method | Fluorometric |
Supplier | Arbor Assays |
Keywords | Oxidative Stress, Stress, Metabolism |
Notes | Glutathione Fluorescent 384-Well Detection assay Kit |
Product Type | Assay & Detection |
Protocol | The DetectX® Glutathione kit is designed to quantitatively measure glutathione (GSH), and oxidized glutathione (GSSG) present in a variety of samples. The kit is unique in that free and oxidized glutathione are detected in a 384-well microtiter plate. No separation or washing is required. Total glutathione is the sum of GSSG plus GSH. Please read the complete kit insert before performing this assay. GSH and GSSG standards are provided to generate standard curves for the assay and all samples should be read off the standard curve. The kit utilizes a proprietary nonfluorescent molecule, ThioStar®, that will covalently bind to the free thiol group on GSH to yield a highly fluorescent product. After mixing the sample or standard with ThioStar® and incubating at room temperature for 15 minutes, the fluorescent product is read at 510 nm in a fluorescent plate reader with excitation at 390 nm. The concentration of the GSH in the sample is calculated, after making a suitable correction for any dilution of the sample, using software available with most fluorescence plate readers. Oxidized glutathione, GSSG, is measured after blocking any GSH in the sample by treatment with 2-Vinylpyridine. GSSG is converted into free GSH using our stable, liquid formulations of NADPH and Glutathione Reductase. The GSH formed then reacts with ThioStar® to yield the signal related to Oxidized GSH content. The total concentration of GSH generated in the sample is calculated from the measured Reduced and Oxidized GSH. We have provided special 384 well plates for measurement but this assay is adaptable for higher density plate formats. The end user should ensure that their HTS black plate is suitable for use with these reagents prior to running samples. |
Sample Type | Blood, Plasma, Serum, Tissue, Urine |
Sensitivity | 45 nM in the Free GSH and 48 nM in the Total GSH assays. |
Shipping Information | BLUE ICE |
Size | 2x384 well plate |
Species Reactivity | cat/feline, cow/bovine, dog/canine, horse/equine, human, mammals, mouse, multi species, pig/porcine, rabbit, rat, sheep/ovine |
Storage | 4°C |
Technical Specifications | Glutathione |
Product Page Updated | 2024-03-04T10:32:24.675Z |