Hemoglobin Colorimetric Detection kit (2 plate)

Article No K013-H1
Product Type Assay & Detection
Application ELISA
Size 2x96 well plates

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Specifications

Country Availability DK, EE, FI, IS, LT, LV, NO, SE
Data Sheet Link http://www.arborassays.com/documentation/inserts/K013-H1.pdf
Description Hemoglobin Colorimetric Detection kit (2 plate)
Supplier Arbor Assays Inc.
Notes The DetectX® Hemoglobin Detection kit uses a single reaction solution that is stable at 4*C, not light sensitive and does not contain dangerous chemicals. All forms of hemoglobin are rapidly converted to a single stable form that is measured photometrically at 560 nm. A human hemoglobin standard is provided to generate a standard curve for the assay. Hemoglobin (Hb) is an erythrocyte protein complex comprised of two sets of identical pairs of subunits, each of which bind an iron-prophyrin group commonly called heme. Generally containing two alpha or alpha-like globulin chains, the remaining subunits may be beta, gamma, delta or epsilon, or in the case of infants, fetal hemoglobin that is replaced during the first year of life. Heme binds and releases oxygen or carbon dioxide in response to slight changes in local gas tension. Free oxygen or carbon dioxide bound by one heme group facilitates subsequent binding by the other heme groups in a given hemoglobin molecule. Subtle changes in pH also regulate hemoglobin affinity for free gases, resulting in a high level of hemostatic control. Hemoglobin values are associated with a variety of conditions ranging from anemias (low Hb), erythrocytosis (high Hb), thalassemias (aberrant chain synthesis) and sickling disorders (abnormal complex shape).
Protocol All samples need to be diluted in ths supplied Hemoglobin Sample Diluent prior to use. Detailed protocols for all samples are included in the kit insert. • Pipet 10 μL of samples or standards into duplicate wells in the microtiter plate. • Add 100 μL of the supplied Hemglobin Detection Reagent to each well. • Incubate at room temperature for 30 minutes. • Read optical density at 560 nm.
Sensitivity 20 µg/ml
Species Reactivity species independent
Storage Stable 4˚C Liquid Reagents
Technical Specifications 5 RBC lysate samples were tested in this kit and in a competitors kit. The linear regression line had a slope of 1.025 an intercept of -0.0565 and a correlation coefficient of 0.9928 Two Formats - Regular Format for Whole Blood and RBCs, and a High Sensitivity Format for Plasma & Serum
Product Page Updated 2019-01-03T09:12:03.477Z

Shipping info

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Species reactivity:

species independent

Product insert:

Product data sheet

Datasheet