Urea Nitrogen (BUN) detection Kit (Two Plates)

Urea Nitrogen (BUN) detection Kit (Two Plates)

Article No K024-H1
Product Type Assay & Detection
Size 2 x 96 well plate

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Country Availability DK, EE, FI, IS, LT, LV, NO, SE
Data Sheet Link http://www.arborassays.com/documentation/inserts/K024-H.pdf
Description Urea Nitrogen (BUN) detection Kit (Two Plates)
Supplier Arbor Assays Inc.
Notes Urea is a by-product of protein metabolism by the liver, and is therefore removed from the blood by the kidneys. Urea freely filters through the glomerulous, but is reabsorbed by the renal tubules in a flow-dependent fashion. The higher the flow rate, the greater amount of urea nitrogen is cleared from circulation and eliminated through the kidneys. As a result, the level of circulating urea nitrogen, along with serum creatinine, serves as a primary measure of kidney function. Normal adult Blood Urea Nitrogen (BUN) levels should be between 7 and 21 mg urea nitrogen per 100 mL blood (mg/dL 1. Azotemia, poor kidney function, will cause elevated BUN levels (≥ 50 mg/dL) and is associated with acute kidney failure or injury, severe acute pancreatitis, congestive heart failure or gastrointestinal bleeding2-5. Azotemia also can occur with dehydration, as a result of alcohol abuse, or high protein diets. Lower than expected BUN levels are usually not clinically predictive, but are primarily associated with liver disease or malnutrition, including malabsorption and low protein diets6. Urine and saliva are considered to be acceptable non-invasive samples for measurement of urea nitrogen7. Serum creatinine is another metabolic waste product freely filtered by the glumerulous, but does not undergo tubular reabsorption. Its steady rate of elimination is frequently used to generate an index or ratio with BUN values for normalized evaluations. Easy to use Serum Creatinine and Urinary Creatinine Detection kits are also available from Arbor Assays (see Related Products).
References 1. Laboratory reference values. Urea nitrogen (BUN). Rochester, Minn.: Mayo Foundation for Medical Education and Research; Nov. 2010. 2. Waiker, SS and JV Bonentre. “Biomarkers for the diagnosis of acute kidney injury.” Nephron Clin. Pract. 2008. 109:c192-c197. 3. Al Mofleh, IA. World J. Gastroent. “Severe acute pancreatitis: pathogenetic aspects and prognostic factors.” 2008. Congestive heart failure. 14(5):675-684. 4. Iglesiase, J. et al. “Predictors of worsening renal function in adult patients with heart failure receiving recombinant human B-type brain natruiretic peptide (nesiritide).” Nephrol. Dial. Transplant. 2006. 21:3458-3465. 5. Mayo Clinic. “Blood urea nitrogen (BUN) tests.” http://www.mayoclinic.com/health/blood-urea nitrogen/MY00373/DSECTION=results 6. Lum, G and S Leal-Khouri. “Significance of low serum urea nitrogen concentrations.”. Clin. Chem. 1989. 35(4):639-640. 7. Akai, T, et al. “Salivary urea nitrogen as an index to renal function: a test strip method”. Clin. Chem. 1983. 29(10):1825-1827.
Sensitivity 0.030 mg/dL
Species Reactivity species independent
Storage All components of this kit should be stored at room temperature until the expiration date of the kit.
Technical Specifications The DetectX® Urea Nitrogen (also called BUN) Detection Kit is designed to quantitatively measure urea nitrogen in a variety of samples. Please read the complete kit insert before performing this assay. A urea nitrogen standard calibrated to NIST reference materials is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Samples are mixed with Color Reagents A and B and incubated at room temperature for 30 minutes. The colored product is read at 450 nm. The concentration of urea nitrogen in the sample is calculated, after making a suitable correction for any dilution, using software available with most plate readers. The results are expressed in terms of mg/dL urea nitrogen. If samples are to be expressed in terms of mg/dL urea, the data can be converted using the multiplier 2.14. Components: Clear 96 well Plates - 2 Plates. Catalog Number X003-2EA Urea Nitrogen Standard - 250 μL Catalog Number. C089-250UL. Urea Nitrogen at 100 mg/dL in a special stabilizing solution. Calibrated to NIST Standard Reference Material Lot Number 912a. Color Reagent A - 15 mL Catalog Number X094-15ML An acidic solution of Color Reagent A. CAUTION: CAUSTIC Color Reagent B - 15 mL Catalog Number X095-15ML An acidic solution of Color Reagent B. CAUTION: CAUSTIC
Product Page Updated 2019-01-03T09:12:03.477Z


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Species reactivity:

species independent

Product insert:

Product data sheet