Article No
KBB-JAVHZR-96
Human AFP/Alpha-fetoprotein ELISA Kit
Human AFP/Alpha-fetoprotein ELISA Kit (96 Tests). Quantitate Human AFP in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.
Assay & Detection
Specifications
| MW | 68678 MW |
| Additional Information | Alpha-fetoprotein (AFP, α-fetoprotein; also sometimes called alpha-1-fetoprotein, alpha-fetoglobulin, or alpha fetal protein) is a protein that in humans is encoded by the AFP gene. The AFP gene is located on the q arm of chromosome 4 (4q25). AFP is the most abundant plasma protein found in the human fetus. Plasma levels decrease rapidly after birth but begin decreasing prenatally starting at the end of the first trimester. Normal adult levels are usually achieved by the age of 8 to 12 months. The function of AFP in adult humans is unknown; however, in rodents it binds estradiol to prevent the transport of this hormone across the placenta to the fetus. The main function of this is to prevent the virilization of female fetuses. As human AFP does not bind estrogen, its function in humans is less clear. |
| Application | ELISA |
| Article No | KBB-JAVHZR-96 |
| Biosite Brand | Biosite ELISA |
| Country Availability | all |
| Conjugation | Unconjugated |
| Cross Reactivity | There is no detectable cross-reactivity with other relevant proteins. |
| Description | Human AFP/Alpha-fetoprotein ELISA Kit (96 Tests). Quantitate Human AFP in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. |
| Supplier | Nordic Biosite |
| Entrez Gene ID | 174 |
| Gene Symbol | AFP |
| Immunogen | Expression system for standard: NS0; Immunogen sequence: R19-V609 |
| Localization | Secreted. |
| Notes | Human AFP/Alpha-fetoprotein ELISA Kit (96 Tests). Quantitate Human AFP in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. |
| Alias Names | Afp|Alpha fetoglobulin|Alpha-fetoglobulin|Alpha fetoprotein|Alpha-fetoprotein|Alpha-1-fetoprotein|FETA|HPAFP |
| Previous Article No | KBB-F0A7BE |
| Product Type | Assay & Detection |
| Protocol | Aliquot 0.1ml per well of 5000pg/ml, 2500pg/ml, 1250pg/ml, 625pg/ml, 313pg/ml, 156pg/ml, 78pg/ml human AFP standard solutions into the pre-coated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernates, cell lysates, serum or plasma( heparin, EDTA) to each empty well. See “Sample Dilution Guideline” above for details. We recommend that each human AFP standard solution and each sample is measured in duplicate. |
| Range | 78 pg/ml - 5,000 pg/ml |
| Sample Type | Cell Culture, Plasma, Serum |
| Sensitivity | <10 pg/ml |
| Shipping Information | BLUE ICE |
| Size | 96wells/kit |
| Species Reactivity | human |
| Storage | -20°C, RT |
| Technical Specifications | For quantitative detection of human AFP in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
| UniProt Number | P02771 |
| Product Page Updated | 2024-04-17T08:14:24.385Z |
