Article No
LS-B14831-50
NCBI Number | 596 |
Application | ELISA, FC, IF, IHC, IP, WB |
Article No | LS-B14831-50 |
Country Availability | SE, FI, DK, NO, FO, GL |
Clone | 100/D5 |
Clone Type | monoclonal |
Concentration | 1 mg/ml |
Conjugation | Unconjugated |
Description | Bcl-2 antibody LS-B14831 is an unconjugated mouse monoclonal antibody to Bcl-2 (BCL2) from human. It is reactive with human, chimpanzee, dog and other species. Validated for ELISA, Flow, IF, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues. |
Recommended Dilution | FC (0.5 - 1 ug/10e6 cells), IF (1 - 2 ug/ml), IHC-P (0.5 - 15 ug/ml), WB (0.5 - 1 ug/ml) |
Supplier | LifeSpan Biosciences |
Entrez Gene ID | 596 |
Gene Symbol | BCL2 |
Immunogen | Amino acids G aa PAPGIFSSQPG (41-54) of the human protein were used as the immunogen for this Bcl-2 antibody. Percent identity by BLAST analysis: Human, Chimpanzee, Cat, Pig, Dog (100%); Gorilla, Orangutan, Bovine, Goat (91%); Galago (82%). |
Isotype | IgG1 k |
Notes | Bcl-2 antibody LS-B14831 is an unconjugated mouse monoclonal antibody to Bcl-2 (BCL2) from human. It is reactive with human, chimpanzee, dog and other species. Validated for ELISA, Flow, IF, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues. |
Alias Names | BCL2, Apoptosis regulator Bcl-2, B-cell CLL/lymphoma 2, B-cell lymphoma protein 2, Bcl-2, PPP1R50 |
Product Type | Antibodies Primary |
Protocol | The applications listed have been tested for the unmodified form of this product. Other forms have not been tested. This antibody recognizes a protein of 25-26kDa, identified as the Bcl-2 alpha oncoprotein. It shows no cross-reaction with Bcl-x or Bax. Expression of Bcl-2 alpha oncoprotein inhibits the programmed cell death (apoptosis). In most follicular lymphomas, neoplastic germinal centers express high levels of the protein, whereas the normal or hyperplastic germinal centers are negative. Consequently, Bcl-2 antibody is valuable when distinguishing between reactive and neoplastic follicular proliferation in lymph node biopsies. It may also be used in distinguishing between those follicular lymphomas that express Bcl-2 and the small number in which the neoplastic cells are negative. ELISA: Order BSA-free format for coating. |
Purification | Protein G Purified |
Shipping Information | RT |
Size | 50 µg |
Source / Host | mouse |
Species Reactivity | dog/canine, human, mammals, pig/porcine |
Storage | 4°C |
Substrate / Buffer | Sterile Solution PBS |
Technical Specifications | Human BCL2 / Bcl-2 |
Product Page Updated | 2024-04-12T06:35:05.844Z |