Article No
LS-B16433-0.5
Mouse Anti-Human IgA Secondary Antibody (clone NI 69 (A89-034) and NI 184 (A89-035))
Human IgA antibody LS-B16433 is an unconjugated mouse monoclonal antibody. Validated for ELISA, IF and IHC. Tested on 20 paraffin-embedded human tissues.
Antibodies Secondary
Specifications
| Application | ELISA, IF, IHC |
| Article No | LS-B16433-0.5 |
| Country Availability | SE, FI, DK, NO, FO, GL |
| Clone | NI 69 (A89-034) and NI 184 (A89-035) |
| Clone Type | monoclonal |
| Conjugation | Unconjugated |
| Description | Human IgA antibody LS-B16433 is an unconjugated mouse monoclonal antibody. Validated for ELISA, IF and IHC. Tested on 20 paraffin-embedded human tissues. |
| Recommended Dilution | IHC-P (10 ug/ml) |
| Supplier | LifeSpan Biosciences |
| Immunogen | Highly purified monoclonal IgA isolated from pooled human serum. |
| Isotype | IgG1 |
| Notes | Human IgA antibody LS-B16433 is an unconjugated mouse monoclonal antibody. Validated for ELISA, IF and IHC. Tested on 20 paraffin-embedded human tissues. |
| Product Type | Antibodies Secondary |
| Protocol | The reactivity of the antiserum is restricted to determinants on the CH2 domain of IgA. It reacts with both subclasses of IgA as tested in hemagglutination, hemagglutination inhibition, indirect binding enzyme immunoassay, competitive inhibition enzyme immunoassay, immunoblotting, immunoprecipitation, latex agglutination assay and histochemistry (Results of an IUIS/WHO collaborative study, Mestecky J. et al. (1996) J. Immunol. Methods 193, 103-148). To identify the presence of IgA in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as radio immuno assay, ELISA, indirect immunoperoxidase and immunofluorescence staining of cytoplasmic IgA, hemagglutination and immunoblotting. The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:50 to 1:200. in ELISA from 1:500 upwards. in Western blotting from 1:1000 upwards. These data should be interpreted as general recommendations only. |
| Purification | Ascites |
| Shipping Information | RT |
| Size | 0.5 mg |
| Source / Host | mouse |
| Species Reactivity | human |
| Storage | -20°C, 4°C |
| Substrate / Buffer | Lyophilized |
| Product Page Updated | 2024-04-12T06:35:05.844Z |
