Article No
LS-B2053-50
MBD3
MBD3 antibody LS-B2053 is an unconjugated mouse monoclonal antibody to MBD3 (aa215-230) from human. It is reactive with human, bat, bovine and other species. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
Antibodies Primary
Specifications
| NCBI Number | 53615 |
| Application | IHC, WB |
| Article No | LS-B2053-50 |
| Country Availability | SE, FI, DK, NO, FO, GL |
| Clone | 106B691 |
| Clone Type | monoclonal |
| Concentration | 1 mg/ml |
| Conjugation | Unconjugated |
| Description | MBD3 antibody LS-B2053 is an unconjugated mouse monoclonal antibody to MBD3 (aa215-230) from human. It is reactive with human, bat, bovine and other species. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues. |
| Recommended Dilution | IHC-P (10 ug/ml), WB (2 ug/ml) |
| Supplier | LifeSpan Biosciences |
| Entrez Gene ID | 53615 |
| Gene Symbol | MBD3 |
| Immunogen | Amino acids 215-230 (CKAFMVTDEDIRKQEE) of human MBD3 protein were used as the immunogen for this antibody. Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Elephant, Panda, Dog, Bovine, Bat, Horse, Pig, Opossum, Turkey, Chicken, Platypus, Xenopus (100%); Marmoset, Mouse, Rat, Hamster, Rabbit, Stickleback, Pufferfish, Zebrafish (94%); Salmon (88%). |
| Isotype | IgG1 |
| Notes | MBD3 antibody LS-B2053 is an unconjugated mouse monoclonal antibody to MBD3 (aa215-230) from human. It is reactive with human, bat, bovine and other species. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues. |
| Alias Names | MBD3 |
| Product Type | Antibodies Primary |
| Protocol | Immunohistochemistry: LS-B2053 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2053 was determined to be 10 ug/ml. |
| Purification | Protein G Purified |
| Shipping Information | RT |
| Size | 50 µg |
| Source / Host | mouse |
| Species Reactivity | chicken, cow/bovine, dog/canine, frog, horse/equine, human, mammals, pig/porcine |
| Storage | -20°C, 4°C |
| Substrate / Buffer | PBS, 0.05% Sodium Azide |
| Technical Specifications | The theoretical molecular weight of human MBD2 (411 amino acids) is 45 kD and human MBD3 (291 amino acids) is 33 kD. Based on sequence identity, this will detect human, mouse, and rat MBD3 (100%), and human and mouse MBD2 (93%). |
| Product Page Updated | 2024-04-12T06:35:05.844Z |
