Article No
LS-C154206-1
GFP
GFP antibody LS-C154206 is a TR-conjugated goat polyclonal antibody to aequorea victoria GFP. Validated for FLISA, IF and WB.
Antibodies Primary
Specifications
| Application | FLISA, IF, WB |
| Article No | LS-C154206-1 |
| Country Availability | SE, FI, DK, NO, FO, GL |
| Clone Type | polyclonal |
| Concentration | 1 mg/ml |
| Conjugation | Texas Red |
| Description | GFP antibody LS-C154206 is a TR-conjugated goat polyclonal antibody to aequorea victoria GFP. Validated for FLISA, IF and WB. |
| Recommended Dilution | FLISA (1:20000), IF (1:500 - 1:2500), WB (1:10000) |
| Supplier | LifeSpan Biosciences |
| Immunogen | Recombinant Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria. |
| Isotype | IgG |
| Notes | GFP antibody LS-C154206 is a TR-conjugated goat polyclonal antibody to aequorea victoria GFP. Validated for FLISA, IF and WB. |
| Alias Names | GFP |
| Product Type | Antibodies Primary |
| Protocol | Polyclonal anti-GFP is designed to detect GFP and its variants. This antibody can be used to detect GFP by ELISA (sandwich or capture) for the direct binding of antigen and recognizes wild type, recombinant and enhanced forms of GFP. Biotin conjugated polyclonal anti-GFP used in a sandwich ELISA is well suited to titrate GFP in solution using this antibody in combination with a monoclonal anti-GFP antibody using either form of the antibody as the capture or detection antibodies. However, use the monoclonal form only for the detection of wild type or recombinant GFP as this form does not sufficiently detect 'enhanced' GFP. The detection antibody is typically conjugated to biotin and subsequently reacted with streptavidin conjugated HRP. Fluorochrome conjugated polyclonal anti-GFP can be used to detect GFP by immunofluorescence microscopy in prokaryotic (E. coli) and eukaryotic (CHO cells) expression systems and can detect GFP containing inserts. Significant amplification of signal is achieved using fluorochrome conjugated polyclonal anti-GFP relative to the fluorescence of GFP alone. For immunoblotting use either alkaline phosphatase or peroxidase conjugated polyclonal anti-GFP to detect GFP or GFP containing proteins on western blots. Optimal titers for applications should be determined by the researcher. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. |
| Purification | Affinity Purified |
| Shipping Information | RT |
| Size | 1 mg |
| Source / Host | goat |
| Species Reactivity | invertebrates |
| Storage | -20°C, 4°C |
| Substrate / Buffer | 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide, 1% BSA |
| Technical Specifications | Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum and purified and partially purified Green Fluorescent Protein (Aequorea victoria) Serum. No reaction was observed against Human, Mouse and Rat Serum Proteins. |
| Product Page Updated | 2024-04-12T06:35:05.844Z |
