Article No
LS-C27178-0.1
Complement C4d
Complement C4d antibody LS-C27178 is an unconjugated mouse monoclonal antibody to human Complement C4d. Validated for ELISA, IF, IHC and WB.
Antibodies Primary
Specifications
| Application | ELISA, IF, IHC, WB |
| Article No | LS-C27178-0.1 |
| Country Availability | SE, FI, DK, NO, FO, GL |
| Clone | 10-11 |
| Clone Type | monoclonal |
| Concentration | 1 mg/ml |
| Conjugation | Unconjugated |
| Description | Complement C4d antibody LS-C27178 is an unconjugated mouse monoclonal antibody to human Complement C4d. Validated for ELISA, IF, IHC and WB. |
| Recommended Dilution | ELISA (1:5000 - 1:20000), IF (1:250 - 1:600), IHC-Fr (1:100 - 1:750) |
| Supplier | LifeSpan Biosciences |
| Immunogen | Native, from human plasma. |
| Isotype | IgG1 |
| Notes | Complement C4d antibody LS-C27178 is an unconjugated mouse monoclonal antibody to human Complement C4d. Validated for ELISA, IF, IHC and WB. |
| Product Type | Antibodies Primary |
| Protocol | Immunohistochemistry: It has been reported that this antibody works very well on acetone-fixed, frozen renal biopsies. Strong staining is observed in the glomeruli and in some cases the peritubular capillaries. Clone 10-11 has given variable results on formalin-fixed, paraffin-embedded sections. It has been observed that pre-treatment with 88% formic acid for 20 minutes at room temperature is beneficial (6). |
| Purification | Protein A Purified |
| Shipping Information | RT |
| Size | 0.1 mg |
| Source / Host | mouse |
| Species Reactivity | human |
| Storage | -20°C |
| Substrate / Buffer | BBS, 0.09% Sodium Azide |
| Technical Specifications | Recognizes the secreted protein complement component 4d (C4d). The presence of C4d in renal peritubular capillaries is a key indicator for acute antibody-mediated rejection [AMR]. C4d was accepted in 2003 into the Banff classification for identification of acute AMR. Is specific for C4d, a marker that can be used in the detection of acute AMR for kidney, heart, pancreas and lung allografts. C4d is regarded as a key marker of antibody-mediated cell injury and humoral rejection. Complement 1 complex cleaves complement 4 (C4) to form C4b and C4a. C4b levels are strictly regulated. Single site cleavage of the C4ba€™s alpha chain by Factor I forms iC4b and blocks C3 convertase, inhibiting opsonization and activation of the classical pathway. This requires C4 binding protein or CR1 as a cofactor. iC4b is further degraded into C4d and C4c. C4ba€™s short half life means that C4d is present in serum at high enough concentrations to make it a useful marker for classical complement activation. Is used to detect the biomarker C4d which has been described as a a€œfootprinta€ of antibody mediated tissue rejection. The internal thioester of C4b becomes exposed during cleavage to C4d and forms a covalent bond with the cell surface. The longer half-life of covalently bound C4d makes it a footprint of complement activation long after weakly bound antibodies have been cleared by the blood stream. C4 has also been linked to susceptibility to systemic lupus erythematosus and rheumatoid arthritis. |
| Product Page Updated | 2024-04-12T06:35:05.844Z |
