Article No
LS-C347207-1
Application | ELISA, ICC, IHC |
Article No | LS-C347207-1 |
Country Availability | SE, FI, DK, NO, FO, GL |
Clone Type | polyclonal |
Conjugation | Biotin |
Description | Human IgG antibody LS-C347207 is a biotin-conjugated pig polyclonal antibody. Validated for DB, ELISA, ICC and IHC. |
Supplier | LifeSpan Biosciences |
Immunogen | Purified normal IgG isolated from pooled human serum. Freund's complete adjuvant is used in the first step of the immunization procedure. |
Notes | Human IgG antibody LS-C347207 is a biotin-conjugated pig polyclonal antibody. Validated for DB, ELISA, ICC and IHC. |
Product Type | Antibodies Secondary |
Protocol | The reactivity of the antiserum is directed to the Fc and Fab subunits of the IgG molecule. It includes a certain degree of reactivity with other immunoglobulins via the common Fab portion. It does not react with any non-Ig protein in human serum, as tested by immunoelectrophoresis and double radial immunodiffusion. In immunocytochemical and immunohistochemical staining to identify and measure IgG, antigen or antibody, at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases. to identify a specific antigen or immune complex using a reference antibody of human origin in the middle layer of the indirect test procedure. As a second step an avidin or streptavidin conjugate of the user's choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labeled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions for histochemical and cytochemical use are usually between 1:100 and 1:500. in ELISA and comparable non-precipitating antibody-binding assays between 1:1000 and 1:10000. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. |
Purification | Purified |
Shipping Information | RT |
Size | 1 ml |
Source / Host | pig/porcine |
Species Reactivity | human |
Storage | -20°C, 4°C |
Substrate / Buffer | Lyophilized from PBS, pH 7.2, No preservatives added |
Technical Specifications | Human IgG, heavy and light chains. |
Product Page Updated | 2024-03-11T11:55:05.043Z |
Volume | 1 ml |