Antibodies Secondary

Sheep Anti-Human IgG3 Secondary Antibody

Sheep Anti-Human IgG3 Secondary Antibody

Article No




Species Reactivity



1 ml



Source / Host


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Application ID
Article No LS-C347505-1
Country Availability SE, FI, DK, NO, FO, GL
Clone 4D5E7
Clone Type polyclonal
Conjugation Unconjugated
Description Sheep Anti-Human IgG3 Secondary Antibody
Supplier LifeSpan Biosciences
Immunogen Purified homogenous IgG3 is isolated from human serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
Notes Human IgG3 antibody LS-C347505 is an unconjugated sheep polyclonal antibody. Validated for ID.
Alias Names ,
Product Type Antibodies Secondary
Protocol The activity of the antiserum is directed to human IgG3 subclass. It does not react with any non-Ig protein in human serum, as tested by immunoelectrophoresis and double radial immunodiffusion. In precipitating techniques as immunoelectrophoresis and double radial immunodiffusion to identify and measure the concentration of IgG3 subclass in serum or other body fluids. Human IgG constitutes about 75% of the total Immunoglobulins in the serum of normal adults. It consists of four subclasses which are distinguished in isotypic heterogeneity of the gamma chains, and in physicochemical, biological and effector properties. The term [Character 201c]subclass[Character 201d] designates the high degree of homology in amino acid sequences and antigenic structure. In normal adult serum, the approximate percentage composition of IgG with respect to its subclasses is: IgG1:65%, IgG2:23%, IgG3:8% and IgG4:4%. All four subclasses appear in both isotypes of the light chain. Normal serum thus contains eight different molecular forms of IgG, the absolute and relative distribution being genetically controlled and resulting from individual responses to the antigenicity of the environment. Antigenic determinants carrying isotypic subclass specificity can be situated on any of the three structurally very similar sub-regions o the gamma chains, known as domains. In IgG, some antigenic determinants are shared by two or three subclasses, but not by all four. Consequently, antibodies to such determinants will not be strictly subclass-specific. For the identification of human IgG3 in cells and tissues by immunofluorescence or immunocytochemical peroxidase techniques, or in body fluids by ELISA and Western blotting type procedures, NORDIMMUNE monoclonal antibody reagents to the individual subclasses are available.
Purification Antiserum
Shipping Information RT
Size 1 ml
Source / Host sheep/ovine
Species Reactivity human
Storage Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Substrate / Buffer Delipidated, heat-inactivated, lyophilized antiserum. No preservative.
Technical Specifications To Human IgG3, subclass specific.
Product Page Updated 2021-06-30T14:59:48.584Z

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The delivery time for this item is approximately 9-18 business days. If other deviations occur, this will be noted on the order confirmation or communicated via email. Please note, we do reserve the right to select the best packaging and shipping method in order to insure the stability of the product and allow efficient order tracking.